| Effects of NYGGF4 knockdown on insulin sensitivity and mitochondrial function in 3T3-L1 adipocytes. | |
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MedLine Citation:
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PMID: 21080215 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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NYGGF4 is a recently discovered gene that is involved in obesity-associated insulin resistance. It has been suggested that mitochondrial dysfunction might be responsible for the development of insulin resistance induced by NYGGF4 overexpression. In the present study, we aimed to define the impact of down-regulating NYGGF4 expression by RNA interference (RNAi) on the insulin sensitivity and mitochondrial function of 3T3-L1 adipocytes. The results revealed that NYGGF4 knockdown enhanced the glucose uptake of adipocytes, which reconfirmed the regulatory function of NYGGF4 in adipocyte insulin sensitivity. However, an unexpected observation was that knockdown of NYGGF4 reduced intracellular ATP concentration and promoted an increase in mitochondrial transmembrane potential (ΔΨm) and reactive oxygen species (ROS) level without affecting mitochondrial morphology or mtDNA. Therefore, the role of NYGGF4 in mitochondrial function remains unclear, and further animal studies are needed to explore the biological function of this gene. |
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Authors:
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Chun-Mei Zhang; Xue-Qi Zeng; Rong Zhang; Chen-Bo Ji; Mei-Ling Tong; Xia Chi; Xi-Ling Li; Jia-Zheng Dai; Min Zhang; Yan Cui; Xi-Rong Guo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-16 |
Journal Detail:
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Title: Journal of bioenergetics and biomembranes Volume: 42 ISSN: 1573-6881 ISO Abbreviation: J. Bioenerg. Biomembr. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-11-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7701859 Medline TA: J Bioenerg Biomembr Country: United States |
Other Details:
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Languages: eng Pagination: 433-9 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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