Document Detail


Effects of N-cadherin overexpression on the adhesion properties of embryonic stem cells.
MedLine Citation:
PMID:  19377287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Constitutive overexpression of N-cadherin in mouse embryonic stem cells led to marked changes in the phenotype and adhesion properties of these cells. The changes included the formation of smaller embryonic bodies, elevated mRNA and total protein levels of N-cadherin, and increased amounts of p120 catenin and connexin-43. N-cadherin cells exhibited decreased attachment to non-cell surfaces, while their adhesiveness to each other and to rat neonatal cardiomyocytes was significantly elevated. The findings suggest that N-cadherin overexpression can facilitate electromechanical integration of stem cells into excitable tissues with endogenously high levels of N-cadherin, such as the heart and brain.
Authors:
Zaruhi Karabekian; Nikki D Gillum; Elissa W P Wong; Narine Sarvazyan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-07-12
Journal Detail:
Title:  Cell adhesion & migration     Volume:  3     ISSN:  1933-6926     ISO Abbreviation:  Cell Adh Migr     Publication Date:    2009 Jul-Sep
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-10-19     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  101469464     Medline TA:  Cell Adh Migr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-10     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cadherins / genetics,  metabolism*
Catenins
Cell Adhesion
Cell Adhesion Molecules / metabolism
Cell Differentiation
Cell Line
Cell Shape
Connexin 43 / metabolism
Embryo, Mammalian / cytology
Embryonic Stem Cells / cytology*,  metabolism*
Mice
Phosphoproteins / metabolism
Rats
Grant Support
ID/Acronym/Agency:
F32 HL087529-03/HL/NHLBI NIH HHS; R01 HL076722/HL/NHLBI NIH HHS; R01 HL076722-02S1/HL/NHLBI NIH HHS; R01 HL076722-03/HL/NHLBI NIH HHS; R01 HL076722-04/HL/NHLBI NIH HHS; R01 HL076722-05/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cadherins; 0/Catenins; 0/Cell Adhesion Molecules; 0/Connexin 43; 0/Phosphoproteins; 0/delta catenin
Comments/Corrections

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