Document Detail


Effects of N-acetylcysteine on matrix metalloproteinase-9 secretion and cell migration of human corneal epithelial cells.
MedLine Citation:
PMID:  22766540     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Matrix metalloproteinase-9 (MMP-9) secreted by corneal epithelial cells has a role in the remodelling of extracellular matrix and migration of epithelial cells. Elevated levels of MMP-9 activity in the ocular surface may be involved in the pathogenesis of corneal diseases. N-acetylcysteine (NAC) has been used to treat corneal diseases, including recurrent epithelial erosions. In this study, its effects on the MMP-9 secretion and human corneal epithelial (HCE) cell migration were evaluated in vitro.
METHODS: Confluent HCE cell cultures were treated with 0-20 mM NAC, and tested for MMP-9 secretion and epithelial cell migration by gelatin zymography and scratch wound assay, respectively. Comparisons between different treatment groups were made using analysis of variance, followed by multiple pairwise comparisons.
RESULTS: Twenty mM NAC inhibited the secretion of MMP-9 significantly. Cell migration, assessed after 24 h of wounding, showed a highly significant dose-dependent inhibitory effect.
CONCLUSIONS: This study shows that NAC reduces MMP-9 production by HCE cells and inhibits cell migration in vitro. This information helps to elucidate the mechanisms by which NAC may be beneficial therapeutically and suggests that NAC may be useful for managing corneal erosions and related conditions.
Authors:
T Ramaesh; K Ramaesh; S C Riley; J D West; B Dhillon
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-06
Journal Detail:
Title:  Eye (London, England)     Volume:  26     ISSN:  1476-5454     ISO Abbreviation:  Eye (Lond)     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-13     Completed Date:  2012-10-31     Revised Date:  2013-08-13    
Medline Journal Info:
Nlm Unique ID:  8703986     Medline TA:  Eye (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1138-44     Citation Subset:  IM    
Affiliation:
Division of Reproductive and Developmental Sciences, Genes and Development Group, University of Edinburgh, Edinburgh, UK.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Acetylcysteine / pharmacology*
Animals
Cell Movement / drug effects*
Cells, Cultured
Coculture Techniques
Dose-Response Relationship, Drug
Epithelium, Corneal / cytology,  drug effects*,  enzymology
Fluorescent Antibody Technique, Indirect
Free Radical Scavengers / pharmacology*
Humans
Matrix Metalloproteinase 9 / metabolism*
Mice
Tissue Donors
Wound Healing
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 616-91-1/Acetylcysteine; EC 3.4.24.35/Matrix Metalloproteinase 9
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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