Document Detail

Effects of Morphology vs. Cell-Cell Interactions on Endothelial Cell Stiffness.
MedLine Citation:
PMID:  21359128     Owner:  NLM     Status:  Publisher    
Biological processes such as atherogenesis, wound healing, cancer cell metastasis, and immune cell transmigration rely on a delicate balance between Cell-Cell and cell-substrate adhesion. Cell mechanics have been shown to depend on substrate factors such as stiffness and ligand presentation, while the effects of Cell-Cell interactions on the mechanical properties of cells has received little attention. Here, we use atomic force microscopy to measure the Young's modulus of live human umbilical vein endothelial cells (HUVECs). In varying the degree of Cell-Cell contact in HUVECs (single cells, groups, and monolayers), we observe that increased cell stiffness correlates with an increase in cell area. Further, we observe that HUVECs stiffen as they spread onto a glass substrate. When we weaken Cell-Cell junctions (i.e., through a low dose of cytochalasin B or treatment with a VE-cadherin antibody), we observe that cell-substrate adhesion increases, as measured by focal adhesion size and density, and the stiffness of cells within the monolayer approaches that of single cells. Our results suggest that while morphology can roughly be used to predict cell stiffness, Cell-Cell interactions may play a significant role in determining the mechanical properties of individual cells in tissues by careful maintenance of cell tension homeostasis.
Kimberly M Stroka; Helim Aranda-Espinoza
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Publication Detail:
Journal Detail:
Title:  Cellular and molecular bioengineering     Volume:  4     ISSN:  1865-5033     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101468590     Medline TA:  Cell Mol Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  9-27     Citation Subset:  -    
Fischell Department of Bioengineering, University of Maryland, College Park, 3138 Jeong H. Kim Engineering Building, College Park, MD 20742, USA.
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Grant Support
F31 NS068028-02//NINDS NIH HHS

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