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The Effects of Mn(2+) on the Proliferation, Osteogenic Differentiation and Adipogenic Differentiation of Primary Mouse Bone Marrow Stromal Cells.
MedLine Citation:
PMID:  23292301     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The effects of Mn(2+) on the proliferation, osteogenic and adipogenic differentiation of BMSCs were evaluated by employing MTT, ΔΨm, cell cycle, ALP activity, collagen production, ARS and oil red O stain assays. The results indicated that Mn(2+) decreased the viability at most concentrations for 24 h, but the viability was increased with prolonging incubation time. Mn(2+) at the concentrations of 1 × 10(-7) and 1 × 10(-6) mol/L decreased ΔΨm in the BMSCs for 48 h. Mn(2+) induced G2/M phase cell cycle arrest at tested concentrations. On day 7 and 10, the effect of Mn(2+) on the osteogenic differentiation depended on concentration, but it inhibited osteogenic differentiation at all tested concentrations for 14 d. The effect of Mn(2+) on the synthesis of collagen of BMSCs depended on concentration for 7 d, but Mn(2+) inhibited the synthesis of collagen at all tested concentrations for 10 d. On day 14, Mn(2+) inhibited the formation of mineralized matrix nodules of BMSCs at all tested concentrations, the inhibitory effect turned to be weaker with prolonging incubation time. Mn(2+) promoted the adipogenic differentiation of BMSCs at all tested concentrations for 10 d, but had no effect with prolonging incubation time. These findings suggested the effects of Mn(2+) on the proliferation, osteogenic differentiation and adipogenic differentiation of BMSCs are very complicated, concentration and incubation time are key factors for switching the biological effects of Mn(2+) from damage to protection.
Authors:
Jinchao Zhang; Qun Zhang; Shenghui Li; Yingjian Hou; Haisong Zhang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-5
Journal Detail:
Title:  Biological trace element research     Volume:  -     ISSN:  1559-0720     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, People's Republic of China, jczhang6970@yahoo.com.cn.
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