| The Effects of Mn(2+) on the Proliferation, Osteogenic Differentiation and Adipogenic Differentiation of Primary Mouse Bone Marrow Stromal Cells. | |
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MedLine Citation:
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PMID: 23292301 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The effects of Mn(2+) on the proliferation, osteogenic and adipogenic differentiation of BMSCs were evaluated by employing MTT, ΔΨm, cell cycle, ALP activity, collagen production, ARS and oil red O stain assays. The results indicated that Mn(2+) decreased the viability at most concentrations for 24 h, but the viability was increased with prolonging incubation time. Mn(2+) at the concentrations of 1 × 10(-7) and 1 × 10(-6) mol/L decreased ΔΨm in the BMSCs for 48 h. Mn(2+) induced G2/M phase cell cycle arrest at tested concentrations. On day 7 and 10, the effect of Mn(2+) on the osteogenic differentiation depended on concentration, but it inhibited osteogenic differentiation at all tested concentrations for 14 d. The effect of Mn(2+) on the synthesis of collagen of BMSCs depended on concentration for 7 d, but Mn(2+) inhibited the synthesis of collagen at all tested concentrations for 10 d. On day 14, Mn(2+) inhibited the formation of mineralized matrix nodules of BMSCs at all tested concentrations, the inhibitory effect turned to be weaker with prolonging incubation time. Mn(2+) promoted the adipogenic differentiation of BMSCs at all tested concentrations for 10 d, but had no effect with prolonging incubation time. These findings suggested the effects of Mn(2+) on the proliferation, osteogenic differentiation and adipogenic differentiation of BMSCs are very complicated, concentration and incubation time are key factors for switching the biological effects of Mn(2+) from damage to protection. |
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Authors:
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Jinchao Zhang; Qun Zhang; Shenghui Li; Yingjian Hou; Haisong Zhang |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-5 |
Journal Detail:
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Title: Biological trace element research Volume: - ISSN: 1559-0720 ISO Abbreviation: Biol Trace Elem Res Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7911509 Medline TA: Biol Trace Elem Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, People's Republic of China, jczhang6970@yahoo.com.cn. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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