| Effects and Mechanism of Integrin-β1 Gene Expression Inhibited by shRNA in Invasion of Pancreatic Carcinoma PANC-1 Cells. | |
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MedLine Citation:
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PMID: 22353524 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background/Aims: To investigate the effects of integrin-β1 gene expression inhibited by shRNA on invasion of pancreatic carcinoma PANC-1 cells in vitro. Methodology The eukaryotic expression plasmid of short hairpin RNA (shRNA) targeting integrin-β1 gene (integrin-β1-shRNA) was constructed and transfected into PANC-1 cells. The expressions of integrin-β1 mRNA and protein were detected by real-time quantitative polymerase chain reaction (PCR) and western blot assay, respectively. The invasive ability of PANC-1 cells was observed with a transwell cell culture chamber and the expressions of MMP-2 and MMP-9 were assayed. Results: Compared to the untransfected group, recombinant expression plasmid integrin-β1-shRNA resulted in reduction of integrin-β1 mRNA and protein by 78.58%±7.24% and 92.88%±3.18%, respectively and the average number of invading PANC-1 cells were decreased from 52±5 to 21±4 (p<0.01) and the expressions of MMP-2 and MMP-9 were inhibited. Conclusions: Recombinant expression plasmid integrin-β1- shRNA can inhibit the expression of integrin-β1 gene and suppress the invasion of PANC-1 cells in vitro significantly. |
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Authors:
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Feng Yu; Hua Li; Xuefeng Bu; Yongjun Zhang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Hepato-gastroenterology Volume: 59 ISSN: 0172-6390 ISO Abbreviation: Hepatogastroenterology Publication Date: 2012 Mar-Apr |
Date Detail:
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Created Date: 2012-02-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8007849 Medline TA: Hepatogastroenterology Country: Greece |
Other Details:
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Languages: eng Pagination: 561-4 Citation Subset: IM |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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