| Effects of MK-196 and furosemide on rat medullary thick ascending limbs of Henle in vitro. | |
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MedLine Citation:
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PMID: 6283059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of the diuretic agents, MK-196 (6,7-dichloro-2-methyl-1-oxo-2-phenyl-5-indanyloxy acetic acid) and furosemide on function of medullary thick ascending limb (tALH) isolated from the rat kidney were studied. tALH were dissected from whole kidneys of male Sprague-Dawley rats which were first perfused in vitro in a recirculating system for 10 to 15 min with a solution containing collagenase. Standard techniques for dissection and perfusion of tALH were followed. Control transepithelial voltage averaged +5.5 +/- 0.5 mV (n = 25). Drug concentration causing 50% inhibition of the voltage (EC50) was determined. EC50 for both MK-196 and furosemide in rat tALH was 8 X 10(-6) M (EC50 for MK-196 in rabbit tALH ws 7 X 10(-5) M). In other experiments, net chloride transport (JCl) in rat tALH averaged 142 +/- 19 pmol.mm-1.min-1 (n = 12), a value nearly twice that reported for the rabbit. In the rat, MK-196 (2 X 10(-4) M) reduced the voltage by 79 +/- 2% and JCl by 76 +/- 13% (n = 6). The same concentration of furosemide inhibited the voltage by 78 +/- 8% and the JCl by 104 +/- 4%(n = 5). Results indicate that: 1) rat tALHs dissected from collagenase-treated kidneys have functional characteristics similar to those reported for the rabbit tALH; and 2) the rat tALH is equally sensitive to MK-196 and furosemide, drugs which act to selectively inhibit active chloride transport. |
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Authors:
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L C Stoner; M E Trimble |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of pharmacology and experimental therapeutics Volume: 221 ISSN: 0022-3565 ISO Abbreviation: J. Pharmacol. Exp. Ther. Publication Date: 1982 Jun |
Date Detail:
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Created Date: 1982-08-26 Completed Date: 1982-08-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376362 Medline TA: J Pharmacol Exp Ther Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 715-20 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chlorides / metabolism Diuretics / pharmacology* Furosemide / pharmacology* Indans / pharmacology* Indenes / pharmacology* Kidney Medulla / drug effects* Kidney Tubules / drug effects* Loop of Henle / drug effects* Male Microbial Collagenase / pharmacology Nephrons / metabolism Rabbits Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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2RO1AM1952/AM/NIADDK NIH HHS; 5RO1AM25695/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Diuretics; 0/Indans; 0/Indenes; 54-31-9/Furosemide; 56049-88-8/indanone; EC 3.4.24.3/Microbial Collagenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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