| Effects of MIP-1 alpha, MIP-3 alpha, and MIP-3 beta on the induction of HIV Gag-specific immune response with DNA vaccines. | |
| | |
MedLine Citation:
|
PMID: 17356539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Transfection of DNA vaccines with chemokines may recruit dendritic cells (DCs) locally to capture the antigenic genes and their gene products to generate enhanced CD8(+) cytotoxic T lymphocytes (CTLs). In this study, we investigated the effects of macrophage inflammatory protein (MIP)-1 alpha, MIP-3 alpha, and MIP-3beta on human immunodeficiency virus (HIV) Gag DNA vaccination. The chemokine plasmids markedly enhanced the local infiltration of inflammatory cells and increased the presence of CD11c(+) B7.2(+)-activated DCs. MIP-1 alpha and MIP-3 alpha were potent adjuvants in augmenting CTLs and afforded strong protection to immunized animals against challenge with vaccinia virus expressing Gag (vv-Gag). However, decreased humoral response was observed. MIP-3beta plasmid did not dramatically alter immunity. The chemokine inoculation time with respect to DNA vaccine priming was also investigated. The injection of pMIP-3 alpha three days before Gag plasmid (pGag) vaccination markedly increased specific CTLs compared with simultaneous injection and led to higher protection against vv-Gag. Immunity was also shifted toward a T-helper type-1 (Th1) response. In contrast, inoculation with pMIP-3 alpha three days after pGag vaccination shifted immunity toward a Th2 response. Our data suggest that administration of a chemokine with DNA vaccines offers a valuable strategy to modulate the efficacy and polarization of specific immunity and that chemokine-antigen timing is critical in determining overall biological effects. |
| | |
Authors:
|
Ruijiang Song; Shuqin Liu; Kam W Leong |
Related Documents
:
|
15246629 - Effect of chemical adjuvants on dna vaccination. 9767429 - Intranasal administration of human immunodeficiency virus type-1 (hiv-1) dna vaccine wi... 19688809 - Fk506 as an adjuvant of tolerogenic dna vaccination for the prevention of experimental ... 10816449 - Dna vaccination against tuberculosis: expression of a ubiquitin-conjugated tuberculosis... 10762079 - Coimmunization with ifn-gamma or il-2, but not il-13 or il-4 cdna can enhance th1-type ... 20097089 - Impact of timing strategy of light, a new tnf superfamily on immune platform induced by... 18378159 - Detection of th1/th2 cytokine signatures in yellow fever 17dd first-time vaccinees thro... 8852409 - Measles immunization in children with a history of egg allergy. 22139689 - Preparation and investigation of ulex europaeus agglutinin i-conjugated liposomes as po... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-03-13 |
Journal Detail:
|
Title: Molecular therapy : the journal of the American Society of Gene Therapy Volume: 15 ISSN: 1525-0016 ISO Abbreviation: Mol. Ther. Publication Date: 2007 May |
Date Detail:
|
Created Date: 2007-04-20 Completed Date: 2007-09-25 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 100890581 Medline TA: Mol Ther Country: United States |
Other Details:
|
Languages: eng Pagination: 1007-15 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology and Molecular Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Chemokine CCL4 Chemotaxis / immunology Dendritic Cells / immunology Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Gene Products, gag / genetics, immunology* HIV / genetics, immunology* Humans Macrophage Inflammatory Proteins / genetics, immunology*, metabolism Mice Mice, Inbred BALB C Microscopy, Fluorescence Plasmids / administration & dosage, genetics Protein Isoforms / genetics, immunology, metabolism T-Lymphocytes, Cytotoxic / immunology Vaccination / methods Vaccines, DNA / administration & dosage, genetics, immunology* |
| Grant Support | |
ID/Acronym/Agency:
|
EB002849/EB/NIBIB NIH HHS; R01 EB002849-01/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Chemokine CCL4; 0/Gene Products, gag; 0/Macrophage Inflammatory Proteins; 0/Protein Isoforms; 0/Vaccines, DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prevention of weight gain in young adults through a seminar-based intervention program.
Next Document: Human embryonic stem cells and gene therapy.