Document Detail


Effects of MCI-186 upon neutrophil-derived active oxygens.
MedLine Citation:
PMID:  17705989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reactions of 3-methyl-1-phenyl-2-pyrazoline-5-one (MCI-186) with hypochlorous acid and superoxide were analysed by spectrophotometry and mass spectrometry. The results were applied to the neutrophil system to evaluate the scavenging activity of neutrophil-derived active oxygen species by MCI-186. MCI-186 reacted rapidly with hypochlorous acid (1 x 10(6) M(-1)s(-1)) to form a chlorinated intermediate, followed by a slow conversion to a new spectrum. MCI-186 consumed 3 moles of hypochlorous acid and did not react with superoxide. The newly synthesized fluorescence probes, 2-[6-(4'-amino)-phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (APF) and 2-[6-(4'-hydroxy)phenoxy-3H-anthen-3-on-9-yl]benzoic acid (HPF) successfully detected neutrophil-derived active oxygens (Setsukinai K, Urano Y, Kakinuma K, Majima HJ, Nagano T. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. J Biol Chem 2003; 278: 3170-3175). The rate constants for the reaction of hypochlorous acid with MCI-186 and fluorescence probes was in the order of MCI-186 > APF > HPF. Fluorescence due to the oxidation of APF and HPF was observed with the stimulated neutrophils. The result that the intensity from APF oxidation was higher than that from HPF oxidation is compatible with reports that APF selectively reacts with hypochlorous acid. Fluorescence due to oxidation of both APF and HPF decreased when the reactions were carried out in the presence of a fluorescence probe and MCI-186 in a dose-dependent manner. These results indicate that MCI-186 effectively scavenges neutrophil-derived hypochlorous acid and other active oxygens.
Authors:
K Sumitomo; N Shishido; H Aizawa; N Hasebe; K Kikuchi; M Nakamura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Redox report : communications in free radical research     Volume:  12     ISSN:  1743-2928     ISO Abbreviation:  Redox Rep.     Publication Date:  2007  
Date Detail:
Created Date:  2007-08-20     Completed Date:  2007-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9511366     Medline TA:  Redox Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  189-94     Citation Subset:  IM    
Affiliation:
Nakatombetsu National Health Insurance Hospital, Nakatombetsu, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antipyrine / analogs & derivatives*,  chemistry,  pharmacology
Chemiluminescent Measurements / methods
Free Radical Scavengers / chemistry,  pharmacology
Humans
Hypochlorous Acid / chemistry*,  metabolism
Luminol / chemistry
Neutrophils / chemistry*,  drug effects,  metabolism
Reactive Oxygen Species / chemistry*,  metabolism
Spectrophotometry / methods
Superoxides / chemistry
Time Factors
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 521-31-3/Luminol; 60-80-0/Antipyrine; 7790-92-3/Hypochlorous Acid; 89-25-8/phenylmethylpyrazolone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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