| Effects of Low Dose Doxycycline and Bisphosphonate Clodronate on Gingival Levels of Matrix Metalloproteinase -9, Interleukin -1B and Alveolar Bone Loss in Diabetic Rats. A Histomorphometric and Immunohistochemical Study. | |
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MedLine Citation:
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PMID: 22220769 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: The bisphosphonates (BPs) and low dose doxycycline (LDD) have been shown to inhibit bone resorption and to improve the levels of pro-inflammatory mediators and destructive enzymes in gingival tissues, respectively. The purpose of this study was to evaluate the effect of mono and combined BP clodronate and LDD therapies in reducing on gingival levels of matrix-metalloproteinase-9 (MMP-9), interleukin- 1β (IL-1β) and alveolar bone loss in diabetic rats. Methods: 50 adults Wistar rats were divided into five study groups as follows: group 1= diabetes control, group 2= diabetes + periodontitis, group 3= diabetes + periodontitis + LDD, group 4= diabetes + periodontitis + clodronate, group 5= diabetes + periodontitis + LDD + clodronate. LDD and clodronate were given as a single agent or as combination therapy during the 7 days of the post-experimental periodontitis period. On day 7, the rats were sacrified and the mobility of the tooth were recorded and block biopsies were removed. The gingival tissues were analyzed histologically and immunohistochemically for expression of MMP-9 and IL-1β. Alveolar bone loss was evaluated morphometrically under a light microscope. Data analysis was performed statistically by Kruskal Wallis and post hoc Tukey tests and Spearman correlation test. Results: Alveolar bone loss was significantly greater in groups 2, 3, 4 and 5 than group 1 (p<0.05), but was not significantly different among groups 2, 3, 4 and 5 (p>0.05). Animals with periodontitis (group 2) expressed significantly higher levels of MMP-9 and IL-1β compared with those without periodontitis (group 1) (p<0.05). MMP-9 expression was significantly lower in group 3 than groups 1, 2 and 5 (p<0.05). IL-1β expression was significantly lower in the groups 1, 3, 4, 5 than 2 (p<0.01) but was not significantly different among the groups 1, 3, 4 and 5. Positive correlations were found between alveolar bone loss and density of inflammation (rho=0. 319, p=0.021) and between MMP-9 and IL-1β (rho= 0.418, p= 0.002), respectively. Conclusion: Our findings suggest that ligature induced periodontitis in diabetic animals results in significantly higher levels of MMP-9 and IL-1β expression in gingiva. The use of mono- and combined clodronate and LDD administrations may significantly reduce either levels of MMP-9 and IL-1β expression. However drug administrations did not affect alveolar bone levels during the study period. |
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Authors:
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Selin P Ozdemir; Bülent Kurtiş; Gülay Tüter; Seyma Bozkurt; Sibel Elif Gültekin; Burcu Sengüven; Keiko Watanabe; Sevtap Aydın |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-5 |
Journal Detail:
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Title: Journal of periodontology Volume: - ISSN: 1943-3670 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000345 Medline TA: J Periodontol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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DDS, PhD. Private Office, Ankara, Turkey. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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