| Effects of low-amplitude, high-frequency vibrations on proliferation and differentiation of SAOS-2 human osteogenic cell line. | |
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MedLine Citation:
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PMID: 19257810 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the work was to understand the consequences of low-amplitude, high-frequency vibrations on proliferation and differentiation of SAOS-2 cells (sarcoma osteogenetic), an osteoblastic and tumorigenic cell line. We realized a bioreactor composed of an eccentric motor that produces a displacement of 11 mm at frequencies between 1 and 120 Hz on a plate connected to the motor. The cultures of SAOS-2 cells were fixed on the plate, and the linear acceleration provoked by the motor to the cultures was measured. We used 30 Hz as stimulating frequency after a preliminary test on the effect of different frequencies on differentiation of cells. Afterward, SAOS-2 cells were stimulated with 30 Hz for different durations, every day for 4 days. The expression of some genes involved in the differentiation process was analyzed first with a reverse transcriptase-polymerase chain reaction and afterward with a real-time polymerase chain reaction on the most expressed genes. Moreover, the proliferation of cells was evaluated. The results suggest a strong increase in the expression of the genes involved in tissue differentiation in the treated groups with respect to the controls. On the other hand, the proliferation seems to be slowed down, so probably the acceleration perceived by the mechanosensors of the cells changes the cellular cycle by blocking the duplication to early differentiate toward bone tissue. |
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Authors:
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Deborah Pr?; Gabriele Ceccarelli; Laura Benedetti; Giovanni Magenes; Maria Gabriella Cusella De Angelis |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Tissue engineering. Part C, Methods Volume: 15 ISSN: 1937-3392 ISO Abbreviation: Tissue Eng Part C Methods Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-17 Completed Date: 2010-02-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: United States |
Other Details:
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Languages: eng Pagination: 669-79 Citation Subset: IM |
Affiliation:
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Dipartimento di Informatica e Sistemistica, University of Pavia , Pavia, Italy. pdebora@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Count Cell Differentiation* Cell Line, Tumor Cell Proliferation Electricity* Electrophoresis Gene Expression Regulation, Neoplastic Humans Osteogenesis / genetics Reverse Transcriptase Polymerase Chain Reaction Software Vibration* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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