Document Detail


The effects of light intensity and spectral quality on growth and shoot initiation in tobacco callus.
MedLine Citation:
PMID:  16659243     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The effects of eight different narrow band-emitting fluorescent lamps (371-750 nm) and four commercial broad band-emitting fluorescent sources upon growth and shoot initiation in tobacco callus (Nicotiana tabacum var. Wisconsin 38) have been characterized. Wavelength and intensity are equally important parameters in determining morphogenic changes. Near ultraviolet light (371 nm) was found to stimulate (0.024 mw/cm(2)) or inhibit (above 0.15 mw/cm(2)) callus growth and shoot initiation, depending on the light intensity. Stimulation of growth and shoot production occurs also in blue light region, but at higher intensity than in the near ultraviolet. Red and far red light (up to 1.7 mw/cm(2)) do not appear to affect callus growth or stimulate shoot initiation. The enhancement of callus growth and the stimulation of shoot initiation are controlled by the same near ultraviolet-absorbing photoreceptor system present in a small enough concentration that it cannot be recognized in the absorption spectrum of the intact tissue. Carotenoids, porphyrins, and phytochrome associated with the high irradiance response do not appear to qualify as the photoreceptor. Flavonoids are possible candidates. Radiation emitted by fluorescent lamps outside the near visible region was determined, and we concluded that energy levels were not sufficient to affect the reported results. The spectral output of several commercial lamps in the visible and near visible regions is such that there could be different effects on growth and development of tissue cultures.
Authors:
M Seibert; P J Wetherbee; D D Job
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  56     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1975 Jul 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  130-9     Citation Subset:  -    
Affiliation:
GTE Laboratories Incorporated, Waltham, Massachusetts 02154.
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