Document Detail

Effects of Lactobacillus rhamnosus GG on the cell growth and polyamine metabolism in HGC-27 human gastric cancer cells.
MedLine Citation:
PMID:  17927509     Owner:  NLM     Status:  MEDLINE    
Previous in vivo studies have suggested that lactobacilli can exert anti-proliferative effects on the gastric epithelium. However, few data are available on their mechanisms of action. The aim of this study was to investigate the effects of increasing concentrations of Lactobacillus rhamnosus strain GG (L. GG) homogenate on cell growth and proliferation [by 3-(4,5 di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, [(3)H]-thymidine incorporation and polyamine biosynthesis] and apoptosis processes (by Bax/Bcl-2 mRNA expression) in HGC-27 human gastric cancer cells. To verify which bacterial fraction was involved in the antiproliferative and proapoptotic effects, the cytoplasm and cell wall extracts were tested separately. HGC-27 cells were sensitive to the apoptotic induction and growth inhibition by increased concentrations of bacterial homogenate. HGC-27 cells were resistant to the bacterial cell wall fractions, whereas increasing cytoplasm fraction concentrations induced evident antiproliferative and proapoptotic actions. These data suggest that cytoplasm extracts could be responsible for L. GG action on HGC-27 cell proliferation.
Francesco Russo; Antonella Orlando; Michele Linsalata; Aldo Cavallini; Caterina Messa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nutrition and cancer     Volume:  59     ISSN:  0163-5581     ISO Abbreviation:  Nutr Cancer     Publication Date:  2007  
Date Detail:
Created Date:  2007-10-11     Completed Date:  2008-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905040     Medline TA:  Nutr Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  106-14     Citation Subset:  IM    
Laboratory of Biochemistry, Scientific Institute for Digestive Diseases, IRCCS Saverio de Bellis, Castellana G, BA, Italy.
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MeSH Terms
Apoptosis / drug effects*
Cell Division / drug effects*
Cell Line, Tumor
Cell Wall / chemistry
Cytoplasm / chemistry
Lactobacillus rhamnosus / physiology*
Polyamines / metabolism*
Probiotics / pharmacology*
Stomach Neoplasms / metabolism*,  microbiology,  prevention & control
Reg. No./Substance:
0/Polyamines; 10028-17-8/Tritium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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