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Effects of the Fas/Fas-L pathway on fluoride-induced apoptosis in SH-SY5Y cells.
MedLine Citation:
PMID:  19852076     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The mechanisms underlying fluoride-induced apoptosis in neurons still remain unknown. To investigate apoptosis, caspase-3 activity, and mRNA expression of Fas, Fas-L, and caspases (-3 and -8) induced by fluoride, human neuroblastoma (SH-SY5Y) cells were incubated with 0, 20, 40, and 80 mg/L sodium fluoride (NaF) for 24 h in vitro. The data show that cell viability in the 40 and 80 mg/L fluoride groups were significantly lower than that of the control group. The percentages of apoptosis in the 40 and 80 mg/L fluoride groups were markedly higher than those in the control group, and they increased with the increase in fluoride concentration. The activity of caspase-3 and mRNA expression levels for Fas, Fas-L, and caspases (-3 and -8) in the 40 and 80 mg/L fluoride groups were significantly higher than those in the control group. An agonistic anti-Fas monoclonal antibody (CH-11) significantly augmented apoptosis induction by fluoride, showing a synergistic effect, while a Fas-blocking antibody (ZB4) partly inhibited fluoride-induced apoptosis of SH-SY5Y cells. The results indicate that fluoride exposure could induce apoptosis in SH-SY5Y cells, and the Fas/Fas-L signaling pathway may play an important role in the process. © 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 86-92, 2011.
Authors:
Bayi Xu; Zhixia Xu; Tao Xia; Ping He; Ping Gao; Weihong He; Ming Zhang; Lijuan Guo; Qiang Niu; Aiguo Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Environmental toxicology     Volume:  26     ISSN:  1522-7278     ISO Abbreviation:  Environ. Toxicol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100885357     Medline TA:  Environ Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  86-92     Citation Subset:  IM    
Copyright Information:
Copyright © 2009 Wiley Periodicals, Inc.
Affiliation:
MOE Key Lab of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, People's Republic of China; Shantou Municipal Center for Disease Control and Prevention, Shantou 515041, Guangdong, People's Republic of China.
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