Document Detail


Effects of L-arginine pretreatment on nitric oxide metabolism and hepatosplanchnic perfusion during porcine endotoxemia.
MedLine Citation:
PMID:  21508091     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Sepsis is accompanied by an increased need for and a decreased supply of arginine, reflecting a condition of arginine deficiency.
OBJECTIVE: The objective was to evaluate the effects of l-arginine pretreatment on arginine-nitric oxide (NO) production and hepatosplanchnic perfusion during subsequent endotoxemia.
DESIGN: In a randomized controlled trial, pigs (20-25 kg) received 3 μg . kg(-1) . min(-1) lipopolysaccharide (LPS; 5 endotoxin units/ng) intravenously and saline resuscitation. l-Arginine (n = 8; 5.3 μmol . kg(-1) . min(-1)) or saline (n = 8) was infused starting 12 h before LPS infusion and continued for 24 h after the endotoxin infusion ended. Whole-body appearance rates, portal-drained viscera (PDV), and liver fluxes of arginine, citrulline, NO, and arginine de novo synthesis were measured by using stable-isotope infusion of [(15)N(2)]arginine and [(13)C-(2)H(2)]citrulline. Hepatosplanchnic perfusion was assessed by using a primed continuous infusion of para-aminohippuric acid and jejunal intramucosal partial pressure of carbon dioxide and was related to systemic hemodynamics.
RESULTS: Arginine supplementation before LPS increased whole-body NO production in the PDV but not in the liver. Furthermore, it increased blood flow in the portal vein but not in the aorta and hepatic artery. During endotoxin infusion, arginine pretreatment was associated with an increased whole-body arginine appearance and NO production in the gut. Additional effects included a preserved mean arterial pressure, the prevention of an increase in pulmonary arterial pressure, an attenuated metabolic acidosis, and an attenuated increase in the intramucosal partial pressure of carbon dioxide.
CONCLUSION: Arginine treatment starting before endotoxemia appears to be beneficial because it improves hepatosplanchnic perfusion and oxygenation during prolonged endotoxemia, probably through an enhancement in NO synthesis, without causing deleterious systemic side effects.
Authors:
Martijn Poeze; Maaike J Bruins; Fons Kessels; Yvette C Luiking; Wouter H Lamers; Nicolaas E P Deutz
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-20
Journal Detail:
Title:  The American journal of clinical nutrition     Volume:  93     ISSN:  1938-3207     ISO Abbreviation:  Am. J. Clin. Nutr.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-23     Completed Date:  2011-07-28     Revised Date:  2013-08-19    
Medline Journal Info:
Nlm Unique ID:  0376027     Medline TA:  Am J Clin Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1237-47     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, Maastricht University Medical Center, Maastricht, Netherlands, Maastricht, Netherlands. m.poeze@ah.unimaas.nl
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MeSH Terms
Descriptor/Qualifier:
Acidosis / prevention & control
Animals
Arginine / deficiency,  pharmacology*,  therapeutic use
Bacteria / chemistry*
Blood Pressure / drug effects
Carbon Dioxide / metabolism
Dietary Supplements
Disease Models, Animal
Endotoxemia / blood,  drug therapy,  metabolism*
Female
Gastrointestinal Tract / metabolism
Lipopolysaccharides
Liver / blood supply,  drug effects*,  metabolism
Mucous Membrane / metabolism
Nitric Oxide / biosynthesis*
Portal Vein / drug effects*
Random Allocation
Splanchnic Circulation / drug effects*,  physiology
Swine
Grant Support
ID/Acronym/Agency:
R01 GM084447/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 10102-43-9/Nitric Oxide; 124-38-9/Carbon Dioxide; 74-79-3/Arginine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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