Document Detail


Effects of Kaempferia parviflora extracts and their flavone constituents on P-glycoprotein function.
MedLine Citation:
PMID:  17031860     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to examine the effects of extracts and flavone derivatives from the rhizome of Kaempferia parviflora on P-glycoprotein (P-gp)-mediated transport in LLC-GA5-COL150, a transfectant cell line of a porcine kidney epithelial cell line LLC-PK1 with human MDR1 cDNA. Ethanol extract obtained from Kaempferia parviflora rhizome significantly increased the accumulation of rhodamine 123 and daunorubicin, P-gp substrates, in LLC-GA5-COL150 cells, but not in LLC-PK1 cells. The aqueous extract also increased the accumulation in LLC-GA5-COL150 cells with lower potency than the ethanol extract. The effects of flavone derivatives isolated from the rhizome of Kaempferia parviflora on P-gp function were examined. Among six flavones tested, 3,5,7,3',4'-pentamethoxyflavone most potently increased the accumulation of rhodamine 123 and daunorubicin in LLC-GA5-COL150 cells in a concentration-dependent manner. In addition, 5,7-dimethoxyflavone to lesser degree increased rhodamine 123 accumulation in LLC-GA5-COL150 cells. In contrast, the other four flavone derivatives had no significant effect on the accumulation of rhodamine 123 in LLC-GA5-COL150 cells in a concentration range tested. These results indicate that extracts and flavone derivatives from the rhizome of Kaempferia parviflora can inhibit P-gp function, which may be useful for overcoming P-gp-mediated multidrug resistance and improving the oral bioavailability of anticancer agents.
Authors:
Denpong Patanasethanont; Junya Nagai; Ryoko Yumoto; Teruo Murakami; Khaetthareeya Sutthanut; Bung-Orn Sripanidkulchai; Chavi Yenjai; Mikihisa Takano
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmaceutical sciences     Volume:  96     ISSN:  0022-3549     ISO Abbreviation:  J Pharm Sci     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-04     Completed Date:  2007-02-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985195R     Medline TA:  J Pharm Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  223-33     Citation Subset:  IM    
Copyright Information:
(c) 2006 Wiley-Liss, Inc. and the American Pharmacists Association.
Affiliation:
Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Hiroshima 734-8553, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibiotics, Antineoplastic / metabolism
Daunorubicin / metabolism
Dose-Response Relationship, Drug
Drug Resistance, Neoplasm / drug effects*
Ethanol / chemistry
Flavones / isolation & purification,  pharmacology*
Flavonoids / pharmacology
Fluorescent Dyes / metabolism
Humans
LLC-PK1 Cells
P-Glycoprotein / drug effects*,  metabolism
Plant Extracts / chemistry,  pharmacology
Rhizome
Rhodamine 123 / metabolism
Solvents / chemistry
Swine
Time Factors
Transfection
Water / chemistry
Zingiberaceae*
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Flavones; 0/Flavonoids; 0/Fluorescent Dyes; 0/P-Glycoprotein; 0/Plant Extracts; 0/Solvents; 20830-81-3/Daunorubicin; 21392-57-4/5,7-dimethoxyflavone; 62669-70-9/Rhodamine 123; 64-17-5/Ethanol; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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