Document Detail

Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells.
MedLine Citation:
PMID:  17391001     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. METHODS: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. RESULTS: Heating of HSC4 cells at 45 degrees C for 20 min and KB cells for 3 min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 microM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. CONCLUSIONS: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.
Keiji Matsuda; Shin-Ya Nakagawa; Tomoya Nakano; Jun-Ichi Asaumi; Ganesh Chandra Jagetia; Shoji Kawasaki
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group     Volume:  22     ISSN:  0265-6736     ISO Abbreviation:  Int J Hyperthermia     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2007-03-29     Completed Date:  2007-04-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8508395     Medline TA:  Int J Hyperthermia     Country:  England    
Other Details:
Languages:  eng     Pagination:  729-35     Citation Subset:  IM    
Division of Radiological Technology, Graduate School of Health Science, Okayama University, Okayama, Japan.
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MeSH Terms
Benzhydryl Compounds / pharmacology*
Cell Line, Tumor
Heat-Shock Proteins / antagonists & inhibitors*
Histones / chemistry*,  drug effects
Hyperthermia, Induced / adverse effects*
KB Cells
Mouth Neoplasms / therapy*
Neoplasms, Squamous Cell / therapy*
Pyrrolidinones / pharmacology*
Reg. No./Substance:
0/Benzhydryl Compounds; 0/Heat-Shock Proteins; 0/Histones; 0/KNK 437; 0/Pyrrolidinones

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