| Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells. | |
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MedLine Citation:
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PMID: 17391001 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. METHODS: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. RESULTS: Heating of HSC4 cells at 45 degrees C for 20 min and KB cells for 3 min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 microM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. CONCLUSIONS: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating. |
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Authors:
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Keiji Matsuda; Shin-Ya Nakagawa; Tomoya Nakano; Jun-Ichi Asaumi; Ganesh Chandra Jagetia; Shoji Kawasaki |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group Volume: 22 ISSN: 0265-6736 ISO Abbreviation: Int J Hyperthermia Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2007-03-29 Completed Date: 2007-04-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8508395 Medline TA: Int J Hyperthermia Country: England |
Other Details:
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Languages: eng Pagination: 729-35 Citation Subset: IM |
Affiliation:
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Division of Radiological Technology, Graduate School of Health Science, Okayama University, Okayama, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Benzhydryl Compounds
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pharmacology* Cell Line, Tumor Heat-Shock Proteins / antagonists & inhibitors* Histones / chemistry*, drug effects Humans Hyperthermia, Induced / adverse effects* KB Cells Methylation Mouth Neoplasms / therapy* Neoplasms, Squamous Cell / therapy* Pyrrolidinones / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Benzhydryl Compounds; 0/Heat-Shock Proteins; 0/Histones; 0/KNK 437; 0/Pyrrolidinones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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