| Effects of IFN-gamma and Stat1 on gene expression, growth, and survival in non-small cell lung cancer cells. | |
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MedLine Citation:
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PMID: 17348819 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stat transcription factors are activated by cytokines and can activate pathways important in oncogenesis. Although previous studies have identified an oncogenic role of Stat3 in lung cancer cells, the role of Stat1 is unclear. Using a mutant of Stat1 with constitutive activity (Stat1C), we examined the effect of persistent Stat1 activity on lung cancer cell growth, survival and gene expression. We identified no significant effect of Stat1C alone or with interferon-gamma (IFN-gamma) on lung cancer cell growth or survival. Consistent with prior reports, Stat1C expression alone elicited minimal changes in gene expression and required costimulatory IFN-gamma for full activity. Using oligonucleotide gene arrays and quantitative real-time PCR, we identified numerous proinflammatory gene products and chemokines regulated by IFN-gamma/Stat1C signaling. These results suggest the major role of IFN-gamma and Stat1 in lung cells is to direct a proinflammatory gene expression program rather than have major effects on cell growth or survival or both. |
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Authors:
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Jiannong Li; Bin Yu; Lanxi Song; Steve Eschrich; Eric B Haura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research Volume: 27 ISSN: 1079-9907 ISO Abbreviation: J. Interferon Cytokine Res. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-12 Completed Date: 2007-05-01 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9507088 Medline TA: J Interferon Cytokine Res Country: United States |
Other Details:
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Languages: eng Pagination: 209-20 Citation Subset: IM |
Affiliation:
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Thoracic Oncology/Experimental Therapeutics, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antiviral Agents / pharmacology* Carcinoma, Non-Small-Cell Lung / genetics, metabolism* Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects, genetics Chemokines / biosynthesis, genetics Gene Expression Regulation, Neoplastic / drug effects*, genetics Humans Inflammation / genetics, metabolism Interferon-gamma / pharmacology* STAT1 Transcription Factor / genetics, metabolism* STAT3 Transcription Factor / genetics, metabolism Signal Transduction / drug effects*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Antiviral Agents; 0/Chemokines; 0/STAT1 Transcription Factor; 0/STAT1 protein, human; 0/STAT3 Transcription Factor; 0/STAT3 protein, human; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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