Document Detail


Effects of high-dose chemotherapy on bone marrow multipotent mesenchymal stromal cells isolated from lymphoma patients.
MedLine Citation:
PMID:  20138957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: High-dose chemotherapy (HDCT) followed by autologous stem cell transplantation is a widely applied treatment for hematological and autoimmune diseases. Little is known about the effects of this therapy on multipotent mesenchymal stromal cells (MSCs). We aimed to characterize, morphologically and functionally, MSCs isolated from bone marrow aspirates of patients after HDCT. MATERIALS AND METHODS: We studied 12 consecutive lymphoma patients submitted to BEAM conditioning regimen followed by autologous stem cell transplantation 28 to 1836 days before the sample collection. Thirteen normal donors were used as control. MSCs were isolated by adherence to plastic and expanded ex vivo by culture in flasks containing alpha-minimum essential medium plus 15% fetal bovine serum. RESULTS: The cell population isolated showed a typical MSC morphology, immunophenotype, and differentiation capacity into adipogenic, osteogenic, and chondrogenic lineages. The MSCs obtained from patients with Hodgkin's disease and non-Hodgkin's lymphoma showed decreased fibroblastoid colony-forming unit count (p = 0.023) and increased doubling time (p = 0.031) related to the control group. The total cell expansion of MSCs from normal subjects was marginally superior to the patient group (p = 0.064). There were no differences in gene expression profile, MSCs plasticity, or hematopoiesis support capability between control and patient group. CONCLUSIONS: Results suggest that HDCT applied to lymphoma patients damaged MSCs, which was demonstrated by their reduced clonogenic potential, doubling time, and cell expansion rates when compared to controls.
Authors:
Karen de Lima Prata; Maristela Delgado Orellana; Gil Cunha De Santis; Simone Kashima; Aparecida Maria Fontes; Rita de C?ssia Viu Carrara; Patricia Vianna Bonini Palma; Luciano Neder; Dimas Tadeu Covas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-06
Journal Detail:
Title:  Experimental hematology     Volume:  38     ISSN:  1873-2399     ISO Abbreviation:  Exp. Hematol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-22     Completed Date:  2010-04-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  292-300.e4     Citation Subset:  IM    
Affiliation:
National Institute of Science and Technology in Stem Cell and Cell Therapy, Center for Cell Therapy and Regional Blood Center, Ribeir?o Preto, Brazil; Faculty of Medicine, University of S?o Paulo, Ribeir?o Preto, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antineoplastic Agents / pharmacology*
Antineoplastic Combined Chemotherapy Protocols*
Bone Marrow Cells / drug effects*
Cells, Cultured
Female
Flow Cytometry
Hodgkin Disease*
Humans
Lymphoma, Non-Hodgkin* / pathology
Male
Mesenchymal Stem Cells / drug effects*
Middle Aged
Reverse Transcriptase Polymerase Chain Reaction
Young Adult
Chemical
Reg. No./Substance:
0/Antineoplastic Agents

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