| Effects of Helicobacter pylori on biological characteristics of gastric epithelial cells. | |
| | |
MedLine Citation:
|
PMID: 22763880 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Infection with Helicobacter pylori (H. pylori) strains is linked to an increased risk of inflammation and gastric cancer. To investigate the effects of H. pylori on biological characteristics of gastric epithelial cells SGC-7901, derived from human adenocarcinoma, morphological appearances of both the pathogen and these cells, as well as features of attachment and internalization were observed by using transmission electron microscopy (TEM). We also investigated cell junctions and invasion by TEM and Transwell Invasion Assay. Cell proliferation and apoptosis were assessed by using chromogenic methylthiazol tetrazolium bromide (MTT) dye and flow cytometry. Three types of H. pylori were observed around, attaching to, or invading tumor cells. Cellular damage was characterized by vacuolar degeneration, dilated endoplasmic reticulum (ER), and reduction of organelles. Cell junctions and cell microvilli reduced or disappeared. H. pylori inhibited cell proliferation, whereas it had no effect on apoptosis. It also promoted gastric carcinoma cell invasion. H. pylori damages cell construction, destroys cell junctions, inhibits cell proliferation, promotes cell invasive ability, and, therefore, might accelerate the malignant progress and metastasis of gastric cancer. |
| | |
Authors:
|
P Wang; J Mei; J Tao; N Zhang; H Tian; G-H Fu |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Histology and histopathology Volume: 27 ISSN: 1699-5848 ISO Abbreviation: Histol. Histopathol. Publication Date: 2012 Aug |
Date Detail:
|
Created Date: 2012-07-05 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: Spain |
Other Details:
|
Languages: eng Pagination: 1079-91 Citation Subset: IM |
Affiliation:
|
Department of Pathology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Telocytes, a distinct type of cell among the stromal cells present in the lamina propria of jejunum.
Next Document: Overexpression of vasoactive intestinal peptide receptors and cyclooxygenase-2 in human prostate can...