Document Detail

Effects of HOE 694--a novel inhibitor of Na+/H+ exchange--on NIH 3T3 fibroblasts expressing the RAS oncogene.
MedLine Citation:
PMID:  8223949     Owner:  NLM     Status:  MEDLINE    
Among the sequelae of ras oncogene expression are intracellular alkalinization and increase of cell volume, both phenomena attributed at least in part to activation of the Na+/H+ exchanger. The present study was performed to elucidate the effects of HOE 694--a novel inhibitor of the Na+/H+ exchanger--on intracellular pH, cell volume, cytoarchitecture and cell proliferation of ras oncogene expressing NIH 3T3 fibroblasts. Following transient exposure of the cells to 20 mmol/l NH3/NH4+, intracellular pH decreases sharply. The following slow realkalinization is completely blocked by 10 mumol/1 HOE 694. Half-maximal inhibition is achieved by 100 nmol/l HOE 694. Cell proliferation is inhibited by HOE 694 with similar potency, whereas the increase in cell volume and cytoskeletal transformation are not prevented, even by 10 mumol/l HOE 694.
E Wöll; M Ritter; F Offner; H J Lang; B Schölkens; D Hüssinger; F Lang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmacology     Volume:  246     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-12-17     Completed Date:  1993-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  269-73     Citation Subset:  IM    
Department of Physiology, Hoechst A.G., Frankfurt, Germany.
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MeSH Terms
3T3 Cells / drug effects*,  metabolism
Cell Division / drug effects
Cytoskeleton / drug effects
Gene Expression*
Genes, ras*
Guanidines / pharmacology*
Hydrogen-Ion Concentration
Sodium-Hydrogen Antiporter / antagonists & inhibitors*
Sulfones / pharmacology*
Reg. No./Substance:
0/Guanidines; 0/Sodium-Hydrogen Antiporter; 0/Sulfones; 141923-47-9/3-methylsulfonyl-4-piperidinobenzoyl guanidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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