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Effects of Fatty acids on human serum albumin binding centers.
MedLine Citation:
PMID:  22866302     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Albumin is a carrier of nonesterified long-chain fatty acids and many other ligands. The status of its binding centers was studied for various proportions of nonesterified long-chain fatty acids and albumin as exemplified by palmitic acid. The status of the binding center was tested by recording K-35 probe fluorescence decay in the subnanosecond band. This method showed the work of three types of centers. Palmitic acid enhanced binding activity of all centers, though to a different degree: if the palmitic acid/albumin proportion increased to 2-3, the probe binding to type 1 centers (located in the drug center I region) increased 1.5 times, while binding to type 3 centers increased more than 3-fold. Modification of these centers by nonesterified long-chain fatty acids was similar in the isolated human albumin preparation and in diluted blood serum. Hence, K-35 probe showed the actual status of various albumin centers, their binding capacity depending to a different measure on the fatty acid charge of albumin.
Authors:
G E Dobretsov; T I Syrejshchikova; N V Smolina; M G Uzbekov
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bulletin of experimental biology and medicine     Volume:  153     ISSN:  1573-8221     ISO Abbreviation:  Bull. Exp. Biol. Med.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-08-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372557     Medline TA:  Bull Exp Biol Med     Country:  United States    
Other Details:
Languages:  eng; rus     Pagination:  323-6     Citation Subset:  IM    
Affiliation:
Institute of Physicochemical Medicine; P. N. Lebedev Physical Institute, the Russian Academy of Sciences, Moscow; Institute of Psychiatry, the Ministry of Health and Social Development, Russia. esmoline@mail.ru.
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