| Effects of FGF9 on embryonic Sertoli cell proliferation and testicular cord formation in the mouse. | |
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MedLine Citation:
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PMID: 15470636 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Proliferation and cord formation by embryonic Sertoli cells are pivotal events involved in testis morphogenesis. A number of growth factors have been implicated in mediating these events. However, the exact level of involvement and importance of each as yet remains elusive. We have adopted an in vitro approach to assess developing mouse Sertoli cells, whereby they are cultured in the presence or absence of fibroblast growth factor (FGF9) and/or extracellular matrix (ECM) gel, since previous studies have shown that ECM gel aids Sertoli cell differentiation. The present findings corroborate this effect, but in addition demonstrate that in the presence of FGF9 (10 ng/ml), cells undergo greater proliferation than those cultured on gel alone. They also display a differentiated epithelial phenotype, with appositional contact of cell membranes in cord-like aggregations. In addition we have shown that cultured Sertoli cells generally express a smaller truncated, nuclear form of the FGFr3, although in the presence of FGF9 and absence of gel, the larger, cytoplasmic form of the receptor is also expressed. Immunolocalisation of FGFr3 in Sertoli cells of whole testes revealed a temporal expression pattern profile, with high levels being abundant in the embryonic testicular cords and at puberty, but an absence in adult Sertoli cells. Our findings suggest that FGF9 plays an important role in proliferation and organisation of embryonic Sertoli cells during testis morphogenesis. |
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Authors:
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Louise Willerton; Robert A Smith; David Russell; Sarah Mackay |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The International journal of developmental biology Volume: 48 ISSN: 0214-6282 ISO Abbreviation: Int. J. Dev. Biol. Publication Date: 2004 Sep |
Date Detail:
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Created Date: 2004-10-07 Completed Date: 2005-04-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8917470 Medline TA: Int J Dev Biol Country: Spain |
Other Details:
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Languages: eng Pagination: 637-43 Citation Subset: IM |
Affiliation:
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Sertoli Cell Group, Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cell Proliferation Cells, Cultured Extracellular Matrix / metabolism Fibroblast Growth Factor 9 Fibroblast Growth Factors / biosynthesis*, physiology* Immunohistochemistry Male Mice Mice, Inbred CBA Microscopy, Phase-Contrast Mitosis Phenotype Sertoli Cells / cytology*, metabolism* Testis / cytology, embryology*, metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Fgf9 protein, mouse; 0/Fibroblast Growth Factor 9; 62031-54-3/Fibroblast Growth Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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