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The Effects of Er:YAG Laser Treatment on Titanium Surface Profile and Osteoblastic Cell activity: An In Vitro Study.
MedLine Citation:
PMID:  21189088     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background: Laser light has been proposed as a tool to decontaminate the surface of endosseous implants. The effects of this maneuver on the interactions between cells and surface however are poorly known. The goal of the present study is to investigate osteoblast growth and differentiation on three commercially available surfaces untreated or after irradiation by Er:YAG laser at two levels, 150 and 200 mJ/pulse at 10 Hz. Methods: human osteoblastic Saos-2 cells were plated on machined (M), sandblasted, acid etched titanium (SAE) or plasma sprayed titanium discs (TPS). The effects of lasing were observed with a scanning electronic microscope (SEM) and cell viability was measured by MTT assay. Moreover, we measured the production of the osteoblast-specific protein osteocalcin (OCN) and of osteoprotegerin (OPG) in the supernatants by immunoenzymatic assays. Results: Although no visible changes were observed on M or TPS samples at the tested levels, titanium peaks on SAE discs appeared fused as a consequence of laser irradiation. Interestingly, cell proliferation was slower on irradiated titanium at both intensities on all the surfaces. Cell differentiation, as assessed by OCN production, was generally unaffected by laser treatement, whereas the production of OPG was decreased on all the surfaces irradiated at the intensity of 200mJ/10Hz. Conclusions: Taken together, these results indicate that Er:YAG laser at energy levels used in this study can alter the surface profile of titanium implants and these changes may negatively affect the viability and the activity of osteoblastic cells, therefore Er:YAG lasers should be used with caution on titanium surfaces.
Authors:
Carlo Galli; Guido M Macaluso; Erida Elezi; Francesca Ravanetti; Antonio Cacchioli; Giacomo Gualini; Giovanni Passeri
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-28
Journal Detail:
Title:  Journal of periodontology     Volume:  -     ISSN:  1943-3670     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Unit of Periodontology, University of Parma, Parma, Italy.
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