| Effects of Dynein on Microtubule Mechanics and Centrosome Positioning. | |
| | |
MedLine Citation:
|
PMID: 22013075 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
In order to determine forces on intracellular microtubules, we measured shape changes of individual microtubules following laser severing. Surprisingly, regions near newly created minus ends increased in curvature following severing, while regions near new microtubule plus ends depolymerized without any observable change in shape. With dynein inhibited, regions near severed minus ends straightened rapidly following severing. These observations suggest that dynein exerts a pulling force on the microtubule which buckles the newly created minus end. Moreover, the lack of any observable straightening suggests that dynein prevents lateral motion of microtubules. To explain these results, we developed a model for intracellular microtubule mechanics which predicts the enhanced buckling at the minus end of a severed microtubule. Our results show that microtubule shapes reflect a dynamic force balance, in which dynein motor and friction forces dominate elastic forces arising from bending moments. A centrosomal array of microtubules subjected to dynein pulling forces and resisted by dynein friction is predicted to center on the experimentally observed timescale, with or without the pushing forces derived from microtubule buckling at the cell periphery. |
| | |
Authors:
|
Jun Wu; Gaurav Misra; Robert J Russell; Anthony J C Ladd; Tanmay P Lele; Richard B Dickinson |
Related Documents
:
|
2835345 - The reliability of estimates of interhemispheric transmission times derived from uniman... 19872535 - Phototropism and the light-sensitive system of phycomyces. 21230895 - Constraining the qcd phase diagram by tricritical lines at imaginary chemical potential. 8475775 - Evaluating the special role of time in the control of handwriting. 8189195 - The structure of attentional control: contingent attentional capture by apparent motion... 20073535 - Spatial composition grading of quaternary zncdsse alloy nanowires with tunable light em... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-19 |
Journal Detail:
|
Title: Molecular biology of the cell Volume: - ISSN: 1939-4586 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
*Department of Chemical Engineering, University of Florida, Gainesville FL 32611. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structural basis for the substrate specificity of a novel {beta}-N-acetyl-hexosaminidase StrH from S...
Next Document: SEQUESTRATION OF PHOSPHOINOSITIDES BY MUTATED MARCKS EFFECTOR DOMAIN INHIBITS STIMULATED CA2+ MOBILI...