| Effects of Different Swimming Exercise Intensities on Bone Tissue Composition in Mice: A Raman Spectroscopy Study. | |
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MedLine Citation:
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PMID: 21182452 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Objective: Raman spectroscopy was employed to evaluate the effect of different swimming intensities on femoral bone composition in an animal model. Background Data: Intense swimming exercise may affect bone mineralization, and Raman technique has been shown to be effective in evaluating tissue composition (phosphate minerals and carbonate apatites - bands at 960 and 1170 cm(-1), as well as collagen matrix - amide I band at 1660 cm(-1)). Materials and Methods: Eighteen female Swiss Webster mice were separated into three groups (n = 6 per group) of sedentary (SED), and swimming with an intensity of 40% (PT-40) and 80% (PT-80) of the maximum load, with 6 weeks of training. Near-infrared Raman spectra (830 nm wavelength and 80 mW laser power) were obtained with a dispersive Raman spectrometer using a CCD camera and imaging spectrograph with 30-s integration time. Spectra were collected in the medial and lateral diaphysis of the femur and principal components analysis (PCA) was employed to extract features of the Raman bands of bone and to perform quantitative analysis. Results: PC1 vector resembles Raman spectra and carries information about apatite minerals and some contribution from organic matrix. A statistically significant difference was found in the PC1 scores (ANOVA, p < 0.05), indicating lower mineral concentrations in the femur in both the PT-40 and PT-80 groups compared to the SED group. These results corroborated with the radiographic assessment of bone density. Conclusion: Raman technique associated with PCA statistics showed that intense swimming exercise may affect bone mineralization and remodeling in a mouse model of training. |
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Authors:
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Fabiano Fernandes da Silva; Renato Aparecido de Souza; Marcos Tadeu Tavares Pacheco; Wellington Ribeiro; Marcos Augusto Souza Rodrigues da Silva; Humberto Miranda; Miguel Angel Castillo Salgado; Julio Cezar de Melo Castilho; Landulfo Silveira |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-12-23 |
Journal Detail:
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Title: Photomedicine and laser surgery Volume: - ISSN: 1557-8550 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101222340 Medline TA: Photomed Laser Surg Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1 Grupo de Estudo e Pesquisa em Ciência da Saúde (GEP-CS), Instituto Federal de Educação, Ciência e Tecnologia do Sul de Minas - Campus Muzambinho , Bairro Morro Preto, Muzambinho, MG, Brazil . |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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