Document Detail


Effects of dietary folic acid on the expression of myelin basic protein in the brain and spinal cord of pregnant and lactating rats.
MedLine Citation:
PMID:  20090312     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Background/Aims: This study investigated the effects of dietary folic acid on the expression of myelin basic protein (MBP) in the maternal brain and spinal cord during pregnancy and lactation. Methods: Female Sprague-Dawley rats were fed either a folic-acid-supplemented diet (FS, 8 mg/kg diet) or a folic-acid-deficient diet (FD, 0 mg/kg diet) from 2 weeks prior to mating until the end of lactation. The expressions of MBP were analyzed using Western blot analysis and immunohistochemistry, and myelin oligodendrocyte glycoprotein (MOG), and neuronal nuclear antigen by immunohistochemistry. Results: The cerebrocortical expression of MBP was 87% higher at day 20 of pregnancy than before pregnancy in FS animals (p < 0.05) but did not change significantly in FD animals. No significant change was observed in the hippocampus or spinal cord in either dietary treatment group. The cerebrocortical MOG and NeuN expressions were significantly lower in FD animals than in FS animals before pregnancy and increased at day 20 of pregnancy but did not differ with the dietary folic acid level. Conclusions: Folic acid deficiency did not increase the expression level of MBP in the cerebral cortex during pregnancy, suggesting that folate intake during pregnancy plays an important role in the maintenance of myelin.
Authors:
Ji Hye Lee; Yo A Lee; Kyung Hee Oh; Namsoo Chang
Publication Detail:
Type:  Journal Article     Date:  2010-01-19
Journal Detail:
Title:  Annals of nutrition & metabolism     Volume:  56     ISSN:  1421-9697     ISO Abbreviation:  Ann. Nutr. Metab.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-05     Completed Date:  2010-05-18     Revised Date:  2011-08-23    
Medline Journal Info:
Nlm Unique ID:  8105511     Medline TA:  Ann Nutr Metab     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  83-90     Citation Subset:  IM    
Copyright Information:
Copyright 2010 S. Karger AG, Basel.
Affiliation:
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Nuclear / drug effects,  metabolism
Blotting, Western / methods
Brain / drug effects,  metabolism*
Cerebral Cortex / drug effects,  metabolism
Diet / methods
Dietary Supplements*
Female
Folic Acid / pharmacology*
Folic Acid Deficiency / metabolism
Lactation / drug effects,  metabolism*
Models, Animal
Myelin Basic Proteins / drug effects,  metabolism*
Myelin-Associated Glycoprotein / drug effects,  metabolism
Pregnancy / metabolism*
Rats
Rats, Sprague-Dawley
Spinal Cord / drug effects,  metabolism*
Vitamin B Complex / pharmacology
Chemical
Reg. No./Substance:
0/Antigens, Nuclear; 0/Myelin Basic Proteins; 0/Myelin-Associated Glycoprotein; 0/myelin oligodendrocyte glycoprotein; 12001-76-2/Vitamin B Complex; 59-30-3/Folic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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