| Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen. | |
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MedLine Citation:
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PMID: 19633681 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of the present study was to determine the effects of docosahexaenoic acid (DHA)-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk (group I), DHA-enriched hen egg yolk (group II), normal hen egg yolk with 5 mmol L(-1) of cysteine supplementation (group III) and DHA-enriched hen egg yolk with 5 mmol L(-1) of cysteine supplementation (group IV). The semen was cryopreserved using controlled rate freezer and was thawed at 50 degrees C for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone (group III) improved progressive motility (P < 0.05), and the supplementation of L-cysteine in combination with DHA-enriched hen egg yolk (group IV) improved both progressive motility (P < 0.05) and acrosome integrity (P < 0.01). The use of DHA-enriched hen egg yolk alone (group II) did not enhance any of the post-thawed semen qualities (P > 0.05). In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity. |
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Authors:
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Panida Chanapiwat; Kampon Kaeoket; Padet Tummaruk |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-27 |
Journal Detail:
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Title: Asian journal of andrology Volume: 11 ISSN: 1745-7262 ISO Abbreviation: Asian J. Androl. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-07 Completed Date: 2009-12-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100942132 Medline TA: Asian J Androl Country: China |
Other Details:
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Languages: eng Pagination: 600-8 Citation Subset: IM |
Affiliation:
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Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / pharmacology Chickens Cryopreservation / methods, veterinary* Cryoprotective Agents / pharmacology* Cysteine / pharmacology* Docosahexaenoic Acids / pharmacology* Egg Yolk* Male Semen / drug effects Semen Analysis Semen Preservation / methods, veterinary* Sperm Motility / drug effects Spermatozoa / drug effects Swine |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Cryoprotective Agents; 25167-62-8/Docosahexaenoic Acids; 52-90-4/Cysteine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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