Document Detail

Effects of D2O on the movement of chromosomes and the shortening of kinetochore spindle fibers in anaphase in dividing spermatocytes of the grasshopper, Mongolotettix japonicus.
MedLine Citation:
PMID:  2776227     Owner:  NLM     Status:  MEDLINE    
Spindles in anaphase of dividing primary spermatocytes of the grasshopper, Mongolotettix japonicus, were examined with a sensitive polarizing microscope combined with a time-lapse video recorder and a cinematographic apparatus. The pole-to-pole distance of the meiotic spindles was increased and the kinetochore fibers were more birefringent in the presence of 40% D2O. However, the rate of shortening of the kinetochore fibers in anaphase was not affected by D2O. This indicates that D2O did not inhibit microtubule disassembly in anaphase, supporting the earlier observations (3, 18) that D2O did not "stabilize" the spindle microtubules at concentrations below 45%. We confirmed that D2O, at the concentration mentioned above, neither promotes nor inhibits the anaphase A. However, the overall sequence of anaphase was considerably extended in the presence of D2O, presumably due to the increased pole-to-pole distance.
S B Sumitro; K Izutsu; H Sato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell structure and function     Volume:  14     ISSN:  0386-7196     ISO Abbreviation:  Cell Struct. Funct.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-10-26     Completed Date:  1989-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7608465     Medline TA:  Cell Struct Funct     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  345-52     Citation Subset:  IM    
Sugashima Marine Biological Laboratory, School of Science, Nagoya University, Mie, Japan.
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MeSH Terms
Anaphase / drug effects*
Chromosomes / drug effects*,  physiology
Deuterium / pharmacology*
Grasshoppers / physiology*
Mitotic Spindle Apparatus / drug effects*,  metabolism,  physiology
Spermatocytes / cytology,  drug effects*,  physiology
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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