Document Detail


Effects of D-penicillamine on growth and cell cycle kinetics of cultured rabbit articular chondrocytes.
MedLine Citation:
PMID:  6712306     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The long-acting antirheumatic drug D-penicillamine was found to inhibit the growth of asynchronous cultures of rabbit articular chondrocytes. This inhibitory effect was dose-related between 5 X 10(-4) M and 5 X 10(-3) M and was time-dependent for a given dose. Flow cytometric analysis showed that drug exposure led to a slowdown in cell cycle progression. This was manifested as a decrease in the number of cells in S phase, due especially to an accumulation of cells in G0 G1 and also to a slight cessation of cell transit through G2 M. Recovery experiments showed that the effect is transitory and reversible. It is suggested that the articular chondrocyte is a target cell for D-penicillamine and that these cells have a D-penicillamine sensitive restriction point in the G0 G1 phase of the cell cycle and to a less extent in the G2 M phase.
Authors:
P Jaffray; X Ronot; M Adolphe; J Fontagne; P Lechat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the rheumatic diseases     Volume:  43     ISSN:  0003-4967     ISO Abbreviation:  Ann. Rheum. Dis.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-05-04     Completed Date:  1984-05-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0372355     Medline TA:  Ann Rheum Dis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  333-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage, Articular / analysis,  cytology,  drug effects*
Cell Cycle / drug effects
Cell Division / drug effects
Cells, Cultured
Clone Cells / drug effects
DNA / analysis
Dose-Response Relationship, Drug
Flow Cytometry
Penicillamine / pharmacology*
Rabbits
Chemical
Reg. No./Substance:
52-67-5/Penicillamine; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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