| Effects of D-3-hydroxybutyrate and acetoacetate on lactate removal in isolated perfused livers from starved and fed rats. | |
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MedLine Citation:
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PMID: 1556952 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the influence of nutritional state on the role of the hepatic plasma membrane lactate transporter in determining overall hepatic lactate disposal. The effects of infusion of sodium D-3-hydroxybutyrate (DOHB) on lactate uptake were studied in perfused livers from fed or starved rats. In livers from starved rats, DOHB (15 to 20 mmol/L) inhibited lactate removal by approximately 45%. This effect was associated with a decrease in intracellular lactate concentration, with cell pH remaining unchanged. Inhibition was maximal when perfusate lactate was less than 1.6 mmol/L, and was undetectable at concentrations exceeding 2.5 mmol/L. A similar degree of inhibition was observed with infusion of acetoacetate. These observations add to the evidence that the inhibition of lactate removal by DOHB seen in livers from starved animals is mediated through an effect on the hepatocyte lactate transporter. At similar low levels of perfusate lactate, DOHB infusion produced a decrease in output of lactate from livers obtained from fed animals. When such livers were subjected to prolonged preperfusion, lactate removal, rather than output, was observed; in these livers DOHB stimulated lactate removal, an effect directionally opposite to that observed in livers from starved animals. These data confirm that hepatic lactate transport is a limiting factor for lactate utilization in intact livers from starved rats; in contrast, lactate utilization in livers from fed animals is limited at a step subsequent to plasma membrane transport, ie, possibly pyruvate transport into mitochondria. |
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Authors:
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H K Metcalfe; J P Monson; F deAllie; R D Cohen |
Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Metabolism: clinical and experimental Volume: 41 ISSN: 0026-0495 ISO Abbreviation: Metab. Clin. Exp. Publication Date: 1992 Apr |
Date Detail:
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Created Date: 1992-05-01 Completed Date: 1992-05-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375267 Medline TA: Metabolism Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 435-40 Citation Subset: IM |
Affiliation:
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Cellular Mechanisms Research Group, London Hospital Medical College, University of London, England. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-Hydroxybutyric Acid Acetoacetates / pharmacology* Animals Eating Hydrogen-Ion Concentration Hydroxybutyrates / pharmacology* Kinetics Lactates / metabolism* Liver / drug effects, metabolism* Perfusion Rats Starvation |
| Chemical | |
Reg. No./Substance:
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0/Acetoacetates; 0/Hydroxybutyrates; 0/Lactates; 300-85-6/3-Hydroxybutyric Acid; 541-50-4/acetoacetic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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