| Effects of Custodiol-N, a novel organ preservation solution, on ischemia/reperfusion injury. | |
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MedLine Citation:
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PMID: 19945120 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Custodiol (histidine-tryptophan-ketoglutarate solution) is a leading organ preservation solution. On the basis of this solution, the novel Custodiol-N was developed. The present study investigated the effects of Custodiol-N in a rat model of heart transplantation. METHODS: Heterotopic heart transplantation was performed in Lewis rats. Four groups were assigned: 2 Custodiol-N-treated groups and 2 Custodiol-treated control groups with a reperfusion time of 1 hour and 24 hours, respectively. Coronary blood flow, left ventricular pressure, its first derivative, left ventricular end-diastolic pressure, endothelium-dependent vasodilatation to bradykinin and endothelium-independent vasodilatation to sodium nitroprusside, and adenosine triphosphate content were measured. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining was performed to detect apoptotic cardiomyocytes. RESULTS: After 1 hour, coronary blood flow (3.99 +/- 0.24 mL/min/g vs 2.86 +/- 0.35 mL/min/g; P < .05), left ventricular pressure (117 +/- 18 mm Hg vs 82 +/- 4 mm Hg; P < .05), and first derivative of left ventricular pressure (3453 +/- 577 mm Hg/s vs 1740 +/- 116 mm Hg/s; P < .05) were significantly higher in the Custodiol-N group compared with the corresponding control. The left ventricular systolic pressure-volume relationship was significantly steeper, indicating improved contractility. Vasodilatatory response to sodium nitroprusside did not show any major differences between the groups. Response to bradykinin resulted in a significantly higher increase in coronary blood flow in the Custodiol-N group (92% +/- 4% vs 60% +/- 5%; P < .05). Myocardial adenosine triphosphate content was significantly higher in the Custodiol-N group (9.84 +/- 0.68 mumol/g vs 1.86 +/- 0.41 mumol/g; P < .05). Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining showed a significantly reduced apoptosis level (21.58% +/- 1.59% vs 27.23% +/- 1.54%; P < .05) in the Custodiol-N group. CONCLUSION: Custodiol-N improves myocardial and endothelial function during the critical phase of reperfusion after heart transplantation. |
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Authors:
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Sivakkanan Loganathan; Tam?s Radovits; Krist?f Hirschberg; Sevil Korkmaz; Achim Koch; Matthias Karck; G?bor Szab? |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-27 |
Journal Detail:
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Title: The Journal of thoracic and cardiovascular surgery Volume: 139 ISSN: 1097-685X ISO Abbreviation: J. Thorac. Cardiovasc. Surg. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-22 Completed Date: 2010-05-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376343 Medline TA: J Thorac Cardiovasc Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1048-56 Citation Subset: AIM; IM |
Copyright Information:
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Copyright 2010 The American Association for Thoracic Surgery. Published by Mosby, Inc. All rights reserved. |
Affiliation:
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Department of Cardiac Surgery, University of Heidelberg, Im Neuenheimer Feld 326 (2.OG), 69120 Heidelberg, Germany. sivakkanan.loganathan@googlemail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Models, Animal Endothelium, Vascular / drug effects* Heart / drug effects* Heart Transplantation* / adverse effects Male Myocardium Organ Preservation Solutions / pharmacology*, therapeutic use Rats Rats, Inbred Lew Reperfusion Injury / etiology, prevention & control* |
| Chemical | |
Reg. No./Substance:
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0/Custodiol-N solution; 0/Organ Preservation Solutions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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