| Effects of cultivation conditions on the production of gamma-PGA with Bacillus subtilis ZJU-7. | |
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MedLine Citation:
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PMID: 18668374 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poly-gamma-glutamic acid (gamma-PGA) is a kind of water-soluble and biodegradable polymer made from D- and L-glutamic acid units, which are linked by amide bonds formed between alpha-amino and gamma-carboxylic acid groups. As a potential targeted biopolymer that can be refined from biomass directly, gamma-PGA has been increasingly applied to food, cosmetic, and pharmaceutical industries. In this work, a suitable nitrogen source was screened out for the high and cost-effective production of gamma-PGA in Bacillus subtilis ZJU-7. The effects of inoculation time and initial glucose concentration on the gamma-PGA production were investigated systematically in both shake flasks and a bench-top 15-l fermentor. Under the optimized culture conditions, a high gamma-PGA productivity (46.4 g/l) was obtained after 48 h cultivation at 37 degrees C. Finally, the large-scale fermentation of gamma-PGA production was successfully scaled up to a 100-l fermentor, with the highest gamma-PGA productivity for over 54.0 g/l. |
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Authors:
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Jie Chen; Feng Shi; Bin Zhang; Fan Zhu; Weifeng Cao; Zhinan Xu; Guohua Xu; Peilin Cen |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-31 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 160 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-26 Completed Date: 2010-04-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 370-7 Citation Subset: IM |
Affiliation:
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Institute of Bioengineering, Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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chemistry,
metabolism* Bioreactors / microbiology Culture Media / chemistry, metabolism Culture Techniques / methods* Fermentation Glucose / metabolism Industrial Microbiology / methods* Polyglutamic Acid / analogs & derivatives*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/poly(gamma-glutamic acid); 25513-46-6/Polyglutamic Acid; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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