Document Detail


Effects of Cryopreservation, Decellularization and Novel ECM Conditioning on the Quasi-static and Time-dependent Properties of the Pulmonary Valve Leaflet.
MedLine Citation:
PMID:  22484150     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Decellularized allografts offer potential as heart valve substitutes and scaffolds for cell seeding. The effects of decellularization on the quasi-static and time-dependent mechanical behavior of the pulmonary valve leaflet under biaxial loading conditions have not previously been reported in the literature. In the current study, the stress-strain, relaxation and creep behavior of the ovine pulmonary valve leaflet was investigated under planar-biaxial loading conditions to determine the effects of decellularization and a novel post-decellularization extracellular matrix (ECM) conditioning process. As expected, decellularization resulted in increased stretch along the loading axes. A reduction in relaxation was observed following decellularization. This was accompanied by a reduction in glycosaminoglycan (GAG) content. Based on previous implant studies, these changes may be of little functional consequence in the short term; however, the long term effects of decreased relaxation and GAG content remain unknown. Some restoration of relaxation was observed following ECM conditioning, especially in the circumferential specimen direction, which may help mitigate any detrimental effects due to decellularization. Regardless of processing, creep under biaxial loading was negligible.
Authors:
Gabriel L Converse; Matt Armstrong; Rachael W Quinn; Eric E Buse; Michael L Cromwell; Sara J Moriarty; Gary K Lofland; Stephen L Hilbert; Richard A Hopkins
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-4
Journal Detail:
Title:  Acta biomaterialia     Volume:  -     ISSN:  1878-7568     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Affiliation:
Cardiac Surgery Research Laboratories of The Ward Family Center for Congenital Heart Disease, Children's Mercy Hospital and Clinics, 2401 Gilham Road, Kansas City, MO 64108.
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