| Effects of CpG-ODNs on phenotype and function of monocyte-derived dendritic cells in chronic hepatitis B. | |
| | |
MedLine Citation:
|
PMID: 22147985 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
AIM: To study the effects of synthetic nonmethylated CpG-containing oligodeoxynucleotides (CpG-ODNs), either alone or combined with recombinant Hepatitis B surface antigen (HBsAg) polypeptide, on the phenotype, function, and intracellular signaling pathways of monocyte-derived dendritic cells (DCs) in patients with chronic hepatitis B (CHB). METHODS: Peripheral blood monocytes isolated from CHB patients and healthy volunteers were induced to be dendritic cells by recombinant human granulocyte-monocyte colony stimulating factor and interleukin-4. The DCs were then treated with CpG-ODNs, CpG-ODNs/HBsAg, or tumor necrosis factor (TNF)-α for 18 h. The expression of surface molecules including HLA-DR, CD86, and CD1a in DCs were detected by flow cytometry, and the expression of signal transducers and activators of transcription (STAT1, 3, 4, 5, 6) and suppressors of cell signaling (SOCS1, 3) were determined by Western blotting assay. In addition, the capacity of DCs to stimulate allogeneic T lymphocytes and the amount of IL-12p70 released from DCs were measured. RESULTS: In the DCs derived from patients with CHB, treatment with TNF-α, CpG-ODNs, or CpG-ODNs/HBsAg, as compared to the vector control, significantly increased the expression of HLA-DR, stimulated the release of IL-12p70, and enhanced the capacity of DCs to stimulate allogenic T lymphocytes. The expressions of STAT1/4/6 and SOCS1/3, but not STAT3/5, were upregulated by TNF-α, CpG-ODNs, and CpG-ODNs/HBsAg. In addition, the expression of CD1a was upregulated only in the presence of both CpG-ODNs and HBsAg. CONCLUSION: The treatment with CpG-ODNs, either alone or combined with HBsAg, has a remarkable stimulatory effect on the impaired phenotype and function of DCs in CHB, possibly by regulating the expression of STAT1, 4, 6 and SOCS1, 3. |
| | |
Authors:
|
Xiao-Xing Xiang; Xia-Qiu Zhou; Jun-Xue Wang; Qing Xie; Xiong Cai; Hong Yu; Hui-Juan Zhou |
Related Documents
:
|
20800785 - The role of vitamin d in asthma. 15332295 - Alternative modalities for asthma that reduce stress and modify mood states: evidence f... 19737225 - Cytokine and anti-cytokine therapy in asthma: ready for the clinic? 21082995 - Leukotriene pathway in sickle cell disease: a potential target for directed therapy. 8047845 - Lipopolysaccharide-stimulated events in b cell activation. 20429415 - The biology of foxp3: a key player in immune suppression during infections, autoimmune ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: World journal of gastroenterology : WJG Volume: 17 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-12-07 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
|
Languages: eng Pagination: 4825-30 Citation Subset: IM |
Affiliation:
|
Xiao-Xing Xiang, Department of Gastroenterology, Northern Jiangsu People's Hospital, Medical College of Yangzhou University, Yangzhou 225001, Jiangsu Province, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A special recurrent pattern in small hepatocellular carcinoma after treatment: Bile duct tumor throm...
Next Document: Simultaneous large cell neuroendocrine carcinoma and adenocarcinoma of the stomach.