| Effects of Clostridium difficile toxins A and B on cytoskeleton organization in HEp-2 cells: a comparative morphological study. | |
| | |
MedLine Citation:
|
PMID: 2515619 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A comparative study on the effects of toxin A and toxin B from Clostridium difficile on HEp-2 cells was carried out. Both toxins caused cell retraction and rounding and seemed to exert their effect on cell morphology via a rearrangement of actin and alpha-actinin microfilaments. Such a rearrangement occurred at an early stage, when no change in microtubular and cytokeratin systems was detectable. Nevertheless, several structural modifications accompanying the cytopathological process induced by toxins A and B appeared to be quite different. In particular, toxin B-treated cells showed an arborized phenotype as a result of cell retraction and rounding, whereas toxin A caused cell rounding without arborization. Moreover, nuclear polarization following disorganization of the microfilament system was only observed in toxin A-treated cells. The structural features distinguishing intoxication processes induced by the two toxins probably reflect a different mechanism of action and suggest the presence of a distinct subcellular component as a primary target for each toxin. |
| | |
Authors:
|
C Fiorentini; G Arancia; S Paradisi; G Donelli; M Giuliano; F Piemonte; P Mastrantonio |
Related Documents
:
|
1926159 - Clostridium difficile toxin a and its effects on cells. 2981969 - Factors involved in interferon-induced or cholera toxin-induced steroidogenesis in y-1 ... 8912639 - Inhibition of adenylyl cyclases by 12(s)-hydroxyeicosatetraenoic acid. 3930519 - Effect of potassium depletion of cells on their sensitivity to diphtheria toxin and pse... 9343949 - Cytotoxicity of anthrax lethal factor microinjected into macrophage cells through senda... 5335899 - Aflatoxin b 1 uptake by flavobacterium aurantiacum and resulting toxic effects. 15378029 - Loss of xiap protein expression by rnai and antisense approaches sensitizes cancer cell... 3736019 - Epithelial-interstitial cell interactions in fetal rat lung development accelerated by ... 2844569 - Participation of mitochondrial proliferation in morphological differentiation of murine... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Toxicon : official journal of the International Society on Toxinology Volume: 27 ISSN: 0041-0101 ISO Abbreviation: Toxicon Publication Date: 1989 |
Date Detail:
|
Created Date: 1990-03-09 Completed Date: 1990-03-09 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 1307333 Medline TA: Toxicon Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 1209-18 Citation Subset: IM |
Affiliation:
|
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Proteins* Bacterial Toxins / isolation & purification, pharmacology* Cells, Cultured Clostridium / analysis Cytoskeleton / drug effects*, ultrastructure Enterotoxins* Epithelial Cells Fluorescent Antibody Technique Humans Microscopy, Electron, Scanning Microscopy, Fluorescence |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Bacterial Toxins; 0/Enterotoxins; 0/tcdA protein, Clostridium difficile; 0/toxB protein, Clostridium difficile |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Elucidation of a role of plasma albumin during collagen-induced aggregation of bovine platelets.
Next Document: The effect of reduced oxygen tension on progesterone accumulation in rat granulosa cell cultures.