Document Detail

Effects of CTLA4-Ig gene-modified dendritic cells on the corneal allografts.
MedLine Citation:
PMID:  16961296     Owner:  NLM     Status:  MEDLINE    
In order to investigate the effects of mouse CTLA4Ig gene-modified dendritic cells (DCs) on the survival of the corneal allografts in rats, the plasmid PG\CTLA4Ig was transfected into DCs of F344 rats mediated by Lipofectamine 2000. The expression of CTLA4Ig was detected by immunofluorescent microscopy. The effects of donor DCs on the proliferation of T cells in Lewis rats (recipients) were tested by by CCK8. Corneal transplantation was performed from F344 rats to Lewis rats. The DCs modified with CTLA4Ig gene were injected into the Lewis rats on the day 0 and 3 after transplantation. The movement of the DCs after modification in vivo was observed by immunofluorescent microscopy, and the survival of corneal allografts was evaluated by Holland criterion. The results showed that the CTLA4Ig-modified DCs could restrain the proliferation of allogenetic T cells. The CTLA4Ig-modified DCs prolonged survival of corneal allografts. (P < 0.01). It was suggested that the injection of CTLA4Ig gene-modified DCs could obviously inhabit the allograft rejection and prolong the survival of corneal allografts.
Bo Han; Yanhua Hu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban     Volume:  26     ISSN:  1672-0733     ISO Abbreviation:  J. Huazhong Univ. Sci. Technol. Med. Sci.     Publication Date:  2006  
Date Detail:
Created Date:  2006-09-11     Completed Date:  2008-06-16     Revised Date:  2013-05-24    
Medline Journal Info:
Nlm Unique ID:  101169627     Medline TA:  J Huazhong Univ Sci Technolog Med Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  366-8     Citation Subset:  IM    
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
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MeSH Terms
Corneal Transplantation / methods*
Dendritic Cells / metabolism,  transplantation*
Graft Rejection / genetics,  immunology
Graft Survival / genetics,  immunology
Immunoconjugates / genetics,  immunology*
Microscopy, Fluorescence
Rats, Inbred F344
Rats, Inbred Lew
Reg. No./Substance:
0/Immunoconjugates; 7D0YB67S97/abatacept

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