Document Detail

Effects of CO(2) pneumoperitoneum on anastomotic healing in rats receiving preoperative 5-fluorouracil neoadjuvant chemotherapy.
MedLine Citation:
PMID:  20001810     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: When used separately, antineoplastic agents and carbon dioxide (CO(2)) pneumoperitoneum have been reported to impair anastomotic healing in experimental animals. However, the effects of their combined use have not been previously investigated. The aim of this study was to investigate the possibility that neoadjuvant chemotherapy with 5-fluorouracil followed by CO(2) pneumoperitoneum would affect the healing of anastomoses in the colon. METHODS: Sprague-Dawley rats (n = 48) were given 5-fluorouracil (20 mg/kg/day) for 5 days, and were then assigned to one of the three groups. Prior to surgery, the control group received no pneumoperitoneum. The other two groups received pneumoperitoneum at 6 and 12 mmHg, respectively, for 2 hr. The large intestine was transected and anastomosis was performed via median laparotomy. On postoperative days 3 and 7, relaparotomy was performed in half of the rats in each group. From the colon, a segment including the anastomosis was excised. Tissue hydroxyproline levels were measured. For histological evaluation, the Verhofstad scale was modified and used. RESULTS: No significant differences in hydroxyproline levels were seen across the groups on postoperative days 3 or 7. However, by postoperative day 7, polymorphonuclear leukocytes and necrosis in the 6-mmHg group had decreased markedly, and granulation had improved. CONCLUSION: Overall, these findings suggest that preoperative 5-fluorouracil therapy followed by pneumoperitoneum at 6 or 12 mmHg does not impair anastomotic healing.
Murat Ulas; Ilter Ozer; Metin Ercan; Yusuf B Ozogul; E Birol Bostanci; Tulay Temucin Keklik; Ummuhani Ozel Turkcu; Ayse Bilgihan; Musa Akoglu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of investigative surgery : the official journal of the Academy of Surgical Research     Volume:  22     ISSN:  1521-0553     ISO Abbreviation:  J Invest Surg     Publication Date:    2009 Nov-Dec
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-04-01     Revised Date:  2010-04-21    
Medline Journal Info:
Nlm Unique ID:  8809255     Medline TA:  J Invest Surg     Country:  England    
Other Details:
Languages:  eng     Pagination:  413-8     Citation Subset:  IM    
Department of Gastroenterological Surgery, Turkiye Yuksek Ihtisas Training and Research Hospital, Ankara, Turkey.
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MeSH Terms
Anastomosis, Surgical
Antimetabolites, Antineoplastic / pharmacology
Carbon Dioxide
Colon / metabolism,  pathology,  surgery*
Fluorouracil / pharmacology*
Hydroxyproline / metabolism
Laparoscopy / methods
Models, Animal
Neoadjuvant Therapy / methods*
Pneumoperitoneum, Artificial / methods*
Rats, Sprague-Dawley
Wound Healing / drug effects*
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 124-38-9/Carbon Dioxide; 51-21-8/Fluorouracil; 51-35-4/Hydroxyproline
Erratum In:
J Invest Surg. 2010 Feb;23(1):66
Note: Dosage error in article text

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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