Document Detail


Effects of BMPs on primary human renal cells and the generation of BMP-7-expressing cells for applications in bioartificial kidneys.
MedLine Citation:
PMID:  21854258     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Bioartificial kidneys (BAKs) contain renal cells, and primary human renal proximal tubule cells (HPTCs) have been applied in clinical trials with BAKs. Cell performance within the device is critical. HPTC performance is often compromised under in vitro conditions due to de-differentiation, trans-differentiation and tubule formation on substrate surfaces. Herein we tested whether treatments with human recombinant bone morphogenetic protein (BMP)-2 or BMP-7 would improve HPTC performance. We found that both growth factors improved HPTC performance, but more consistent results were obtained with BMP-7. The effects were strongly concentration-dependent, and for BMP-7, 25 ng/ml was the optimal concentration, which improved HPTC performance under static and under bioreactor conditions. As an alternative to supplementation with the purified growth factor, we generated HPTCs secreting human recombinant BMP-7. BMP-7 secreted by the cells was bioactive and improved the functional performance of HPTCs, in agreement with our other findings. Together, the results suggested that either supplementation with purified BMP-7 or BMP-7-producing cells could be used to improve cell performance in BAKs. BAKs with BMP-7-producing cells could also be used to deliver the growth factor to kidney patients. Our results suggested that the amounts of BMP-7 produced by HPTCs would be sufficient for therapeutic applications.
Authors:
Farah Tasnim; Karthikeyan Kandasamy; Joscha S Muck; Mohammed Shahrudin Bin Ibrahim; Jackie Ying; Daniele Zink
Related Documents :
22111158 - Sickle cell trait and sudden death.
22331808 - Effects of tirapazamine on experimental colorectal liver metastases after radiofrequenc...
21804408 - Isoflurane preconditioning protects astrocytes from oxygen and glucose deprivation inde...
21783528 - Extruding auditory hair cells in rats exposed to subchronic 3,3'-iminodipropionitrile.
6706228 - Establishment of human endometrial adenocarcinoma cell line containing estradiol-17 bet...
25383178 - A review of adipocyte lineage cells and dermal papilla cells in hair follicle regenerat...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-20
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
home, , #14-405 , 4 pandan valley, singapore, Singapore, Singapore, 597628, +6594220340; farah@ibn.a-star.edu.sg.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Antigenotoxic ketosteroid from the red algae Jania adhaerens.
Next Document:  A Single-Center Experience with Prone Retroperitoneoscopic Versus Open Renal Surgery in Children: An...