| Effects of Aqueous Extracts of Raw Pu-erh Tea and Ripened Pu-Erh Tea on Proliferation and Differentiation of 3T3-L1 Preadipocytes. | |
| | |
MedLine Citation:
|
PMID: 23027678 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Pu-erh tea has shown anti-obesity effects but little is known about its effect on proliferation and differentiation of preadipocytes. This study investigated the effects of the aqueous extracts of raw pu-erh tea and ripened pu-erh tea on proliferation and differentiation of murine 3T3-L1 preadiopocytes. We examined dose and time effects of both aqueous extracts on proliferation of 3T3-L1 preadipocytes. The contents of triglycerides in cytoplasm and the mRNA expression of critical transcriptional factors involved in differentiation were determined. Cytotoxicity and apoptosis rate of preadipocytes by pu-erh tea extracts treatment were test for toxic and pro-apoptotic effects. Both aqueous extracts of pu-erh tea inhibited the proliferation of 3T3-L1 preadipocytes at the selected time points. At lower concentration of raw pu-erh tea extracts (less than 300 µg/ml) and ripened pu-erh tea extracts (less than 350 µg/ml), no significant cytotoxic and pro-apoptotic were observed. Ripened pu-erh tea was more effective with lower IC(50) than raw pu-erh tea. Both extracts suppressed the differentiation and down-regulated the gene expression of peroxisome proliferator-activated receptor-γ and CCAAT/enhancer binding proteins-α. Therefore, these results indicate that both aqueous extracts of pu-erh tea can inhibit proliferation and differentiation with ripened pu-erh tea more potent. Polyphenol rich in both extracts may play a role in the inhibition of proliferation and differentiation of 3T3-L1 preadipocytes. Copyright © 2012 John Wiley & Sons, Ltd. |
| | |
Authors:
|
Zhen-Hui Cao; Hui Yang; Zhan-Long He; Cheng Luo; Zhi-Qiang Xu; Da-Hai Gu; Jun-Jing Jia; Chang-Rong Ge; Qiu-Ye Lin |
Related Documents
:
|
7507228 - The mouse tie receptor tyrosine kinase gene: expression during embryonic angiogenesis. 11599048 - Glucose transporter expression is developmentally regulated in in vitro derived bovine ... 22991308 - The edible red alga, gracilaria verrucosa, inhibits lipid accumulation and ros producti... 10707848 - Apoptosis and expression of proliferative proteins in the developing nervous system and... 8367538 - Inhibition of phthalocyanine-sensitized photohemolysis of human erythrocytes by quercetin. 20130358 - Distribution pattern of gprc6a mrna in mouse tissue by in situ hybridization. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-2 |
Journal Detail:
|
Title: Phytotherapy research : PTR Volume: - ISSN: 1099-1573 ISO Abbreviation: Phytother Res Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-2 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8904486 Medline TA: Phytother Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 John Wiley & Sons, Ltd. |
Affiliation:
|
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, P.R. China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A process-based approach to characterizing the effect of acute alprazolam challenge on visual paired...
Next Document: Measurement Invariance of the Perceived Stress Scale and Latent Mean Differences across Gender and T...