| The effects of alginate encapsulation on NIT-1 insulinoma cells: viability, growth and insulin secretion. | |
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MedLine Citation:
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PMID: 20023235 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transplantation of microencapsulated insulin-secreting cells is proposed as a promising therapy for the treatment of type I diabetes mellitus. In recent years, important advances have been made in the field of immunoisolation and many studies have shown that alginate provides some major advantages for encapsulation over other systems. Since it is known that the extracellular matrix influences the behaviour of encapsulated cells, the aim of the present work has been to study the consequences of encapsulation on some cell functions. For this purpose, cell growth and dynamics of insulin release of NIT-1 cells entrapped in alginate capsules compared with those exhibited by free NIT-1 cells were investigated by means of growth curves, assays, Trypan blue staining and ELISA test. All investigations performed allowed us to conclude that alginate-entrapped NIT-1 cells maintain their growth features and secretory functions although with some important differences. In particular, alginate encapsulation affects the cellular growth profile and causes the lost of time dependence of insulin secretion profile. |
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Authors:
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Alessia Bertolotti; Massimiliano Borgogna; Angelica Facoetti; Eleonora Marsich; Rosanna Nano |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: In vivo (Athens, Greece) Volume: 23 ISSN: 0258-851X ISO Abbreviation: In Vivo Publication Date: 2009 Nov-Dec |
Date Detail:
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Created Date: 2009-12-21 Completed Date: 2010-03-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8806809 Medline TA: In Vivo Country: Greece |
Other Details:
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Languages: eng Pagination: 929-35 Citation Subset: IM |
Affiliation:
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Department of Nuclear, Universit? degli Studi di Pavia, Pavia, Italy. BertolottiAlessia@libero.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alginates
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chemistry,
pharmacology* Animals Biocompatible Materials / chemistry, pharmacology* Cell Line, Tumor Cell Survival / drug effects Cell Transplantation / methods* Drug Carriers / chemistry, pharmacology* Drug Compounding Fungal Proteins Glucuronic Acid / chemistry, pharmacology Hexuronic Acids / chemistry, pharmacology Insulin / secretion Insulinoma / drug therapy*, pathology, secretion Islets of Langerhans / drug effects, pathology, secretion Mice Trans-Activators |
| Chemical | |
Reg. No./Substance:
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0/Alginates; 0/Biocompatible Materials; 0/Drug Carriers; 0/Fungal Proteins; 0/Hexuronic Acids; 0/Trans-Activators; 11061-68-0/Insulin; 144590-62-5/NIT-4 protein, Neurospora crassa; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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