Document Detail

Effects of Akt signaling on nuclear reprogramming.
MedLine Citation:
PMID:  19032345     Owner:  NLM     Status:  MEDLINE    
Reprogramming of the epigenetic state from differentiated to pluripotent cells can be attained by cell fusion of differentiated somatic cells with embryonic stem (ES) cells or transfer of the nucleus of a differentiated cell into an enucleated oocyte. Activation of Akt signaling is sufficient to maintain pluripotency of ES cells and promotes derivation of embryonic germ (EG) cells from primordial germ cells (PGCs). Here we analyzed the effects of Akt signaling on somatic cell nuclear reprogramming after cell fusion and nuclear transfer. We found that forced activation of Akt signaling stimulated reprogramming after cell fusion of ES cells with thymocytes or mouse embryonic fibroblasts. These hybrid cells showed ES cell characteristics, including in vitro and in vivo differentiation capacity. In contrast, Akt signaling significantly reduced the efficiency of reprogramming with nuclear transfer. Our results demonstrate that Akt signaling plays important roles on the nuclear reprogramming of somatic cells.
Toshinobu Nakamura; Kimiko Inoue; Shoko Ogawa; Hiroki Umehara; Narumi Ogonuki; Hiromi Miki; Tohru Kimura; Atsuo Ogura; Toru Nakano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-19
Journal Detail:
Title:  Genes to cells : devoted to molecular & cellular mechanisms     Volume:  13     ISSN:  1365-2443     ISO Abbreviation:  Genes Cells     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-10-08     Completed Date:  2009-11-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9607379     Medline TA:  Genes Cells     Country:  England    
Other Details:
Languages:  eng     Pagination:  1269-77     Citation Subset:  IM    
Graduate School of Frontier Bioscience, Osaka University, Suita, Osaka, Japan.
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MeSH Terms
Cell Differentiation
Cell Line
Enzyme Activation
Nuclear Reprogramming*
Nuclear Transfer Techniques
Proto-Oncogene Proteins c-akt / genetics,  metabolism*
Signal Transduction*
Reg. No./Substance:
EC Proteins c-akt

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