Document Detail


Effects of ACTH administration on zonation of the guinea pig adrenal cortex.
MedLine Citation:
PMID:  10022770     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experiments were done to determine the actions of ACTH on the morphologic and functional characteristics of the zona fasciculata (ZF) and zona reticularis (ZR) in the guinea pig adrenal cortex. In control guinea pigs, a number of morphologic differences distinguished the ZF from the ZR, including the presence of far more lipid in the ZF than in the ZR. Treatment with ACTH decreased the lipid droplet content of the ZF cells, equalizing the amount of lipid in the two zones. Other morphologic differences between the ZF and ZR were also diminished by ACTH treatment. Immunohistochemical analyses indicated that CYP17 protein was found in both the ZF and ZR in control animals, but with greater immunostaining intensity in the ZF. The enzyme protein distribution corresponded with higher 17alpha-hydroxylase activity in the ZF than in the ZR. After ACTH treatment, the intensity of staining and enzyme activities in the two zones were similar, attributable largely to increases in the ZR. In situ hybridization-and immunohistochemistry showed that in control animals CYPD216 was highly expressed in the ZR but not in the ZF. ACTH treatment dramatically reduced the intensity of CYP2D16 mRNA and protein staining in the ZR. Bufuralol 1'-hydroxylase activity, a marker for CYP2D subfamily members, was also decreased significantly in the ZR by ACTH treatment. The data indicate that administration of ACTH to guinea pigs has opposite effects on the expression of CYP17 and CYP2D16 in the ZR, and diminishes or eliminates some of the structural and functional differences between the ZF and ZR. The results suggest a role for ACTH in establishing and maintaining adrenocortical zonation.
Authors:
B B Yuan; R Tchao; Y Funae; J M Voigt; H D Colby
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  146     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-05-03     Completed Date:  1999-05-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  129-36     Citation Subset:  IM    
Affiliation:
Albany College of Pharmacy, NY 12208, USA.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Cortex / anatomy & histology*,  drug effects*,  enzymology
Adrenocorticotropic Hormone / pharmacology*
Animals
Aryl Hydrocarbon Hydroxylases*
Cytochrome P-450 Enzyme System / analysis,  genetics
Guinea Pigs
Immunohistochemistry
Male
RNA, Messenger / analysis
Steroid 17-alpha-Hydroxylase / analysis,  metabolism
Tissue Distribution
Zona Fasciculata / anatomy & histology,  drug effects,  enzymology
Zona Reticularis / anatomy & histology,  drug effects,  enzymology
Grant Support
ID/Acronym/Agency:
AG 11987/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 9002-60-2/Adrenocorticotropic Hormone; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/cytochrome P-450 CYP2D16 (guinea pig); EC 1.14.99.9/Steroid 17-alpha-Hydroxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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