| Effects of 60 Hz magnetic field exposure on polymorphonuclear leukocyte activation. | |
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MedLine Citation:
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PMID: 10572957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated the effects of a sinusoidal 60 Hz magnetic field on free radical (superoxide anion) production, degranulation (beta-glucuronidase and lysozyme release) and viability in human neutrophils (PMNs). Experiments were performed blindly in very controlled conditions to examine the effects of a magnetic field in resting PMNs and in PMNs stimulated with a tumor promoter: phorbol 12-myristate 13-acetate (PMA). Exposure of unstimulated human PMNs to a 60 Hz magnetic field did not affect the functions examined. In contrast, exposure of PMNs to a 22 milliTesla (mT), 60 Hz magnetic field induced significant increases in superoxide anion (O2-) production (26.5%) and in beta-glucuronidase release (53%) when the cells were incubated with a suboptimal stimulating dose of PMA. Release of lysozyme and lactate dehydrogenase was unchanged by the magnetic field, whether the cells were stimulated or not. A 60 Hz magnetic field did not have any effect on O2- generation by a cell-free system xanthine/xanthine oxidase, suggesting that a magnetic field could upregulate common cellular events (signal transduction) leading to O2- generation and beta-glucuronidase release. In conclusion, exposure of PMNs to a 22 mT, 60 Hz magnetic field potentiates the effect of PMA on O2- generation and beta-glucuronidase release. This effect could be the result of an alteration in the intracellular signaling. |
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Authors:
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R Khadir; J L Morgan; J J Murray |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1472 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1999 Oct |
Date Detail:
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Created Date: 1999-11-30 Completed Date: 1999-11-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 359-67 Citation Subset: IM |
Affiliation:
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Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232-0111, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Apoptosis Cell Degranulation Cell-Free System Chemiluminescent Measurements Glucuronidase / metabolism Humans Magnetics* Neutrophil Activation* Neutrophils / drug effects, enzymology, metabolism, radiation effects* Superoxides / metabolism Tetradecanoylphorbol Acetate / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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GM15431/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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11062-77-4/Superoxides; 16561-29-8/Tetradecanoylphorbol Acetate; EC 3.2.1.31/Glucuronidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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