Document Detail

Effects of 5-fluorouracil on mitogen-induced costimulatory capacity of accessory cells from rat oral mucosa and dental pulp.
MedLine Citation:
PMID:  11459322     Owner:  NLM     Status:  MEDLINE    
The present study was designed to investigate the effect of the antimetabolite 5-fluorouracil (5-FU) on the capacity of the oral epithelium and the dental pulp to induce a mitogen-driven T-cell proliferation. Inbred Lewis rats were given 6 i.v. injections of 5-FU (30 mg/kg or 50 mg/kg) over a period of 8 days. Suspensions of oral epithelial and dental pulpal cells were prepared. The costimulatory capacity of the accessory cells from treated animals was monitored by their ability to induce a mitogen (ConA)-mediated proliferation of T cells isolated from regional lymph nodes of untreated animals. Accessory epithelial cells from rats treated with the high dose of 5-FU, but not the low dose, induced a decreased T-cell proliferation compared to controls. Accessory pulpal cells from rats, treated with 30 mg/kg or 50 mg/kg of 5-FU, induced a lower T-cell proliferation. When MHC class II molecule depleted T-cell suspensions from lymph nodes of 5-FU-injected animals were incubated with ConA, a significant proliferative response was observed. This finding correlated with an increase of MHC class II molecule expressing cells detected after incubation, although no such cells were observed immediately following the initial purification step of T cells. This finding demonstrates that the accessory cells could partly restore their expression of MHC class II molecules during incubation. The results of the study suggest that the function of immunocompetent cells of the oral mucosa and dental pulp is influenced by treatment with 5-FU and that the function of accessory cells of the pulp is affected more than the function of accessory cells derived from the oral mucosa.
I von Bültzingslöwen; G Carlsson; B Gustavsson; M Jontell
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  30     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-07-18     Completed Date:  2001-09-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  362-7     Citation Subset:  D; IM    
Department of Endodontology and Oral Diagnosis, Faculty of Odontology, Göteborg University, Sweden.
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MeSH Terms
Antigen-Presenting Cells / drug effects*,  immunology
Antimetabolites / administration & dosage,  pharmacology*
Cell Division / drug effects
Concanavalin A / pharmacology
Dental Pulp / cytology,  drug effects*
Epithelial Cells / drug effects
Fluorouracil / administration & dosage,  pharmacology*
Histocompatibility Antigens Class II / immunology
Immunocompetence / immunology
Injections, Intravenous
Lymph Nodes / cytology
Lymphocyte Activation / drug effects
Mitogens / administration & dosage,  pharmacology*
Mouth Mucosa / cytology,  drug effects*
Rats, Inbred Lew
Statistics as Topic
T-Lymphocytes / drug effects,  immunology
Reg. No./Substance:
0/Antimetabolites; 0/Histocompatibility Antigens Class II; 0/Mitogens; 11028-71-0/Concanavalin A; 51-21-8/Fluorouracil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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