Document Detail


Effects of 5-fluorouracil and 2 alpha-methyldihydrotestosterone propionate on the growth of human breast carcinoma MCF-7 in vitro.
MedLine Citation:
PMID:  6688585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
5-Fluorouracil (5-FU) and 2 alpha-methyldihydrotestosterone propionate (MDTP) have effectively induced complete regressions of induced rat mammary carcinomas; in combination, regressions were additive and synergistic. Present aims were to determine whether similar antitumor effects were obtainable with a human mammary carcinoma, MCF-7, and to affirm the synergism of 5-FU and MDTP. After incubation in vitro for 3 days and exposure to drug for another 2 days, cell counts and/or determinations of total cell protein revealed growth inhibitions of 16-87% by 5-FU at 130-1300 micrograms/ml and 16-94% by MDTP at 0.36-360.5 micrograms/ml. Combinations of 5-FU and MDTP at the same inhibitory doses (ID) yielded approximately additive growth inhibitions. Algebraic and geometric (isobole) methods of analyses showed that these inhibitions were additive or synergistic, depending on the iso-effective dose used. Precursor incorporation into macromolecules also showed approximately additive effects for MCF-7 cells treated with 5-FU and MDTP, each at ID15. These data demonstrate significant additive growth-inhibitory activity of 5-FU and MDTP in combination against MCF-7 in vitro, thus affirming their antitumor effects in vivo.
Authors:
H Fujita; M N Teller; S Green; W Kreis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  European journal of cancer & clinical oncology     Volume:  19     ISSN:  0277-5379     ISO Abbreviation:  Eur J Cancer Clin Oncol     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8112045     Medline TA:  Eur J Cancer Clin Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1231-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Androstanols / administration & dosage,  analogs & derivatives*,  therapeutic use
Breast Neoplasms / drug therapy*,  pathology
Cell Division / drug effects
Cell Line
Dose-Response Relationship, Drug
Drug Interactions
Drug Therapy, Combination
Fluorouracil / administration & dosage,  therapeutic use*
Humans
Grant Support
ID/Acronym/Agency:
CA 08748/CA/NCI NIH HHS; CA 18856/CA/NCI NIH HHS; CA23906/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Androstanols; 51-21-8/Fluorouracil; 521-12-0/dromostanolone propionate; 58-19-5/dromostanolone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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