Document Detail


Effects of 3,4-Dihydroxyacetophenone on the Hypercholesterolemia-induced Atherosclerotic Rabbits.
MedLine Citation:
PMID:  23412669     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
3,4-Dihydroxyacetophenone(3,4-DHAP) is one herbal extract from bald Mao-dong-qing leaves. We reported that 3,4-DHAP had anti-inflammatory function by decreasing tumor necrosis factor-α(TNF-α) secretion in macrophages. The aim of the study was to examine the effects of 3,4-DHAP on plasma and liver lipids, plasma alanine aminotranferase (ALT) and TNF-α level, vascular cell adhesion molecule-1(VCAM-1) expression, plaque vulnerability and vascular inflammation in hypercholesterolemia-induced atherosclerotic rabbits. Male New Zealand white rabbits were randomized into negative control, positive control, 3,4-DHAP and simvastatin groups. From weeks 2 to 12, the rabbits were treated with 3,4-DHAP or simvastatin. At weeks 12, all the animals were sacrificed. Plasma lipids and ALT were measured using the enzymatic endpoint method. Plasma TNF-α was measured using enzyme-linked immuno sorbent assay (ELISA). Liver lipids concentrations were estimated using commercial kits. The expression of VCAM-1 was measured using reverse transcription-polymerase chain reaction (RT-PCR) and Western bolt. Histological analysis was used to evaluate the pathologic changes of rabbit aortas. The results showed that 3,4-DHAP markedly lowered plasma and liver lipids, lowered plasma ALT and TNF-α levels compared with the positive control group. VCAM-1 mRNA and protein were markedly inhibited by 3,4-DHAP. Decreased aortic plaque instability was evident in 3,4-DHAP-treated rabbits, as demonstrated by a thickened elastic layer, increased vascular smooth muscle cells(VSMCs) accumulation in the plaques, less neointima hyperplasia and macrophages recruitment. 3,4-DHAP may attenuate the progression of atherosclerotic lesions and stabilize plaques by lowering plasma lipids, the number of macrophages and the expression of VCAM-1, while increasing the number of VSMCs in the atherosclerotic plaques.
Authors:
Daijuan Zhang; Jiangyue Liu; Lin Wang; Jianying Wang; Wentao Li; Baoxiang Zhuang; Jianping Hou; Tongmei Liu
Related Documents :
24575129 - Evaluation of serum 25-hydroxyvitamin d as a predictor of carotid intima-media thicknes...
9688869 - Effects of daytime melatonin infusion in young adults.
14578589 - Effect of zaleplon, a non-benzodiazepine hypnotic, on melatonin secretion in rabbits.
381589 - Melatonin in humans physiological and clinical studies.
25249019 - Slc30a3 and sep15 gene polymorphisms influence the serum concentrations of zinc and sel...
11697449 - Trends in the musk xylene concentrations in plasma samples from the general population ...
18045669 - Free tryptophan/large neutral amino acids ratios in blood plasma do not predict cerebra...
3583969 - Ciprofloxacin in plasma and peritoneal dialysate after oral therapy in patients on cont...
2113859 - Physiological role of neuropeptide y (npy) in control of anterior pituitary hormone rel...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-15
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  -     ISSN:  1347-5215     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of pathophysiology, Weifang medical college.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Anti-Diabetic Action of 7-O-Galloyl-D-sedoheptulose, a Polyphenol from Corni Fructus, through Amelio...
Next Document:  Histopathologic study of the rectum in 1,438 consecutive rectal specimens in a single Japanese hospi...