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Effects of 28 days of resistance exercise and consuming a commercially available pre-workout supplement, NO-Shotgun(R), on body composition, muscle strength and mass, markers of satellite cell activation, and clinical safety markers in males.
MedLine Citation:
PMID:  19656392     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
PURPOSE: This study determined the effects of 28 days of heavy resistance exercise combined with the nutritional supplement, NO-Shotgun(R), on body composition, muscle strength and mass, markers of satellite cell activation, and clinical safety markers.
METHODS: Eighteen non-resistance-trained males participated in a resistance training program (3 x 10-RM) 4 times/wk for 28 days while also ingesting 27 g/day of placebo (PL) or NO-Shotgun(R) (NO) 30 min prior to exercise. Data were analyzed with separate 2 x 2 ANOVA and t-tests (p < 0.05).
RESULTS: Total body mass was increased in both groups (p = 0.001), but without any significant increases in total body water (p = 0.77). No significant changes occurred with fat mass (p = 0.62); however fat-free mass did increase with training (p = 0.001), and NO was significantly greater than PL (p = 0.001). Bench press strength for NO was significantly greater than PL (p = 0.003). Myofibrillar protein increased with training (p = 0.001), with NO being significantly greater than PL (p = 0.019). Serum IGF-1 (p = 0.046) and HGF (p = 0.06) were significantly increased with training and for NO HGF was greater than PL (p = 0.002). Muscle phosphorylated c-met was increased with training for both groups (p = 0.019). Total DNA was increased in both groups (p = 0.006), while NO was significantly greater than PL (p = 0.038). For DNA/protein, PL was decreased and NO was not changed (p = 0.014). All of the myogenic regulatory factors were increased with training; however, NO was shown to be significantly greater than PL for Myo-D (p = 0.008) and MRF-4 (p = 0.022). No significant differences were located for any of the whole blood and serum clinical chemistry markers (p > 0.05).
CONCLUSION: When combined with heavy resistance training for 28 days, NO-Shotgun(R) is not associated with any negative side effects, nor does it abnormally impact any of the clinical chemistry markers. Rather, NO-Shotgun(R) effectively increases muscle strength and mass, myofibrillar protein content, and increases the content of markers indicative of satellite cell activation.
Authors:
Brian Shelmadine; Matt Cooke; Thomas Buford; Geoffrey Hudson; Liz Redd; Brian Leutholtz; Darryn S Willoughby
Publication Detail:
Type:  Journal Article     Date:  2009-08-05
Journal Detail:
Title:  Journal of the International Society of Sports Nutrition     Volume:  6     ISSN:  1550-2783     ISO Abbreviation:  J Int Soc Sports Nutr     Publication Date:  2009  
Date Detail:
Created Date:  2009-08-25     Completed Date:  2011-07-14     Revised Date:  2011-11-21    
Medline Journal Info:
Nlm Unique ID:  101234168     Medline TA:  J Int Soc Sports Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  16     Citation Subset:  -    
Affiliation:
Department of Health, Human Performance, and Recreation, Baylor University, Box 97313, Waco, TX 76798, USA. darryn_willoughby@baylor.edu.
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