Document Detail

Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on fetal mouse urinary tract epithelium in vitro.
MedLine Citation:
PMID:  11311455     Owner:  NLM     Status:  MEDLINE    
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), produces hydronephrosis by altering the differentiation and proliferation of ureteric epithelial cells in the fetal C57BL/6N mouse urinary tract. This study tests the hypothesis that the late fetal urinary tract epithelial cells respond to TCDD with increased proliferation and that the responses do not require contributions from other maternal or fetal tissues. This was achieved by exposing late gestation fetal urinary tract cells to TCDD in an in vitro model. Isolated ureteric cells from gestation day (GD) 18 fetal ureters were plated in medium supplemented with trace elements, a complex mixture of lipids, a defined mixture of purified hormones and growth factors. Both epithelial and mesenchymal cells remain viable under these conditions. The cultures were exposed to 0.1% dimethylsulfoxide (DMSO), 1x10(-8), 1x10(-9) or 1x10(-10) M TCDD. Exposure to 1x10(-10) M TCDD did not affect the cultures, while 1x10(-8) and 1x10(-9) M TCDD supported epithelial, but not mesenchymal, cell survival and stimulated epithelial cell proliferation and differentiation. The TCDD-exposed cells expressed high levels of keratin and little or no vimentin, confirming that the cells, which survive and differentiate are epithelial. However, after continuous exposure to epidermal growth factor (EGF), the TCDD-induced stimulation of ureteric epithelial growth could not be detected. In conclusion, this study demonstrates that late gestational ureteric cells respond to TCDD in vitro with the stimulation of epithelial cell growth and differentiation.
P L Bryant; L M Reid; J E Schmid; A R Buckalew; B D Abbott
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Toxicology     Volume:  162     ISSN:  0300-483X     ISO Abbreviation:  Toxicology     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-20     Completed Date:  2001-05-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0361055     Medline TA:  Toxicology     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  23-34     Citation Subset:  IM    
Environmental Sciences and Engineering Department, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
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MeSH Terms
Abnormalities, Drug-Induced / etiology*,  metabolism,  pathology
Bromodeoxyuridine / metabolism
Cell Differentiation / drug effects
Cell Division / drug effects
Epidermal Growth Factor / pharmacology
Epithelial Cells / pathology
Mice, Inbred C57BL
Teratogens / metabolism,  toxicity*
Tetrachlorodibenzodioxin / metabolism,  toxicity*
Urinary Tract / drug effects*,  embryology*,  pathology
Grant Support
Reg. No./Substance:
0/Teratogens; 1746-01-6/Tetrachlorodibenzodioxin; 59-14-3/Bromodeoxyuridine; 62229-50-9/Epidermal Growth Factor

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