Document Detail


Effects of (1R,9S)-beta-hydrastine on l-DOPA-induced cytotoxicity in PC12 cells.
MedLine Citation:
PMID:  15044037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
(1R,9S)-beta-Hydrastine in lower concentrations of 10-50 microM inhibits dopamine biosynthesis in PC12 cells. In this study, the effects of (1R,9S)-beta-hydrastine on L-DOPA (L-3,4-dihydroxyphenylalanine)-induced cytotoxicity in PC12 cells were investigated. (1R,9S)-Hydrastine at concentrations up to 250 microM did not reduce cell viability. However, at concentrations higher than 500 microM it caused cytotoxicity in PC12 cells, as determined with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, TUNEL (terminal deoxynucleotidyltransferase dUTP nick-end labeling) method and flow cytometry. Exposure of PC12 cells to cytotoxic concentrations of (1R,9S)-beta-hydrastine (500 and 750 microM) in combination with L-DOPA (20, 50 and 100 microM) after 24 or 48 h resulted in a significant decrease in cell viability compared with the effects of (1R,9S)-beta-hydrastine or L-DOPA alone, and apoptotic cell death was observed. However, the decrease in cell viability induced by (1R,9S)-beta-hydrastine was not prevented by the antioxidant N-acetyl-L-cysteine, indicating that it is not mediated by membrane-based oxygen free radical damage. These data suggest that (1R,9S)-beta-hydrastine has a mild cytotoxic effect and at higher concentration ranges aggravates L-DOPA-induced cytotoxicity in PC12 cells.
Authors:
Shou Yu Yin; Jae Joon Lee; Yu Mi Kim; Chun Mei Jin; Yoo Jung Yang; Min Hee Kang; Masaaki Kai; Myung Koo Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmacology     Volume:  488     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-26     Completed Date:  2004-11-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  71-7     Citation Subset:  IM    
Affiliation:
College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University, San 48, Kaeshin-Dong, Heungduk-Gu, Cheongju 361-763, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / pharmacology
Alkaloids / pharmacology*
Animals
Antioxidants / pharmacology
Apoptosis / drug effects
Benzylisoquinolines
Cell Death / drug effects
Cell Survival / drug effects
DNA Fragmentation / drug effects
Dopamine Agents / pharmacology*
Flow Cytometry
Free Radical Scavengers / pharmacology
Isomerism
Levodopa / pharmacology*
PC12 Cells
Rats
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Antioxidants; 0/Benzylisoquinolines; 0/Dopamine Agents; 0/Free Radical Scavengers; 0/Levodopa; 118-08-1/hydrastine; 616-91-1/Acetylcysteine

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